目的:探讨脂蛋白脂酶(lipoprotein lipase,LPL)基因PvuⅡ酶切多态性与2型糖尿病的相关性。方法:采用聚合酶链反应-限制性片段长度多态性(PGR-RFLP)方法,分析了156例样本LPL基因第6内含子PvuⅡ多态性(病例组98人。对照组58。其中40个2型糖尿病同胞对,病例组40人,对照组40人)。结果:病例组与对照组的基因型和基因频率均无显著性差异。结论:湖北汉族人群脂蛋白脂酶基因PvuⅡ酶切多态性与2型糖尿病无明显关联。
Objective: To study the relationship between the lipoprote, in lipase gene Pvu Ⅱ polymorphism and type 2 diabetes meillitus. Methods: PCR- RFLP method was used to determine the PvuH polymorphism of intron 6 in LPL gene in 156 samples[compatriot group(n = 80 ) : case group(n= 40 ) and control group(n= 40 ) ; randomly selected group (n= 76) : case group ( n=58) and control group ( n = 18) ].Results: There were no significant differences in the genotype and Sere frequency between cases and controls. Condusion: The PvuⅡ polymorphism of LPL gene is not associated with type 2 diabetes mellitus in Hubei Han people.