中文摘要：

Kazrin 最近作为功能的蛋白质被识别了涉及 cellcell 连接并且在信号 transduction。这里，我们识别了新 isoform， Kazrin F，它是在长度的 518 aa 并且有在 N 终点唯一的 97 aa。用在房间生存能力的引起的房间 apoptosis 和显著减少由 MTT 和 TUNEL 试金测量了的 siRNA 的 Kazrin F 击倒。Co-immunoprecipitation 分析表明 Kazrin F 与弧(有 caspase 招募领域的 apoptosis 抑压者) 和 Bax (Bcl-2-associated X 蛋白质) 交往。有在细胞质的弧和 Bax 的 Kazrin F 的合作本地化被 immunofluorescence 分析决定。这些结果建议 Kazrin F 可能在由与弧和 Bax 交往调整细胞的 apoptosis 起一个重要作用。

英文摘要：

Kazrin has recently been identified as a functional protein that is involved in cell-cell junctions and in signal transduction. Here, we identified a new isoform, Kazrin F, which is 518 aa in length and has 97 aa unique at the N-terminus. Knockdown of Kazrin F using siRNA caused cell apoptosis and a marked decrease in cell viability measured by MTT and TUNEL assays. Co-immunoprecipitation analysis revealed that Kazrin F interacts with ARC （apoptosis repressor with caspase recruitment domain） and Bax （Bcl-2-associated X protein）. Co-localization of Kazrin F with ARC and Bax in the cytoplasm was determined by immunofluorescence analysis. These results suggested that Kazrin F might play an important role in regulating cellular apoptosis by interacting with ARC and Bax.