中文摘要：

为了探讨MKI67在肝癌细胞发生发展中的作用,采用实时定量PCR方法检测人肝细胞癌QGY-7703细胞中MKI67基因表达水平,以及MKI67在肝细胞癌组织和癌旁正常组织中的表达情况,设计并合成针对MKI67的siRNA,利用脂质体转染法将其转入QGY-7703细胞内,通过MTT和细胞集落形成实验观察MKI67-siRNA对QGY-7703细胞生长活性和增殖能力的影响.实时定量PCR结果表明,MKI67在肝细胞癌组织中的表达水平明显高于癌旁正常组织（P〈0.01）.MTT和细胞集落形成实验结果显示,转染MKI67-siRNA的QGY-7703细胞生长活性和集落形成率明显低于对照组（P〈0.01）.由此得出结论：MKI67在肝癌细胞系QGY-7703细胞中的表达水平较高,且它在肝癌组织中的表达水平明显上调.同时,MKI67-siRNA可以有效抑制QGY-7703细胞的生长活性和增殖能力,提示MKI67可能与肝细胞癌的发生、发展相关.

英文摘要：

To explore the expression of MKI67 in human hepatocellular carcinoma（HCC）,real-time-PCR（RT-PCR） was used to detect the mRNA expression level of MKI67 in human HCC tissues,the adjacent normal tissues,and human HCC cell line QGY-7703.The chemical synthesized MKI67-siRNA,which was designed to target MKI67,was transfected into QGY-7703 cells.MTT and colony formation assays were used to detect the cell growth activity and proliferation capacity of QGY-7703 cells.The result showed that the expression level of MKI67 mRNA in HCC tissues was higher than that in adjacent normal tissues（P 〈0.01）.MTT and colony formation analyses demonstrated that the cell growth activity and clone formation ratio of MKI67-siRNA transfected QGY-7703 cells were markedly decreased（P〈 0.01）.In conclusion,the MKI67,which was up-regulated in HCC tissues and QGY-7703 cells,promote the cell activity and proliferation ability of QGY-7703 cells.In other word,MKI67 may be related to the pathogenesis of HCC.