中文摘要：

氧化石墨烯（GO）纳米片具有一些优点，如一个大的表面，良好的生物相容性，和简单的化学修饰能力。他们也有伟大的潜力作为药物的载体。在这篇文章中，我们去纳米标记99mTc，满足临床前和临床通过改进的Hummers法合成了纳米research.go MicroSPECT成像探针潜在的需求，然后再与叠氮基团共价功能化纳米片在两步结合守卫遗迹（1,4,7,10-tetraazacyclododecane-n，N，N，乙二醇双乙胺醚-n），通过点击化学手段炔基官能化。通过增加和减少的^ 99mTc锝，守卫遗迹去合成attained.dota-conjugated GOS与500–600纳米的横向尺寸。两原子力显微镜（AFM）和红外光谱进行了表征go-dota.labeling效率与^ 99mTc go-dota＞90%，放射化学纯度〉 96%我们进行纯化。成功合成了氧化石墨烯的衍生物，守卫遗迹共轭GOS，通过点击化学反应，并且它被标记^ SPECT成像99mTc标记效率高。使去片适合平台为未来的分子影像学研究。

英文摘要：

Graphene oxide（GO）nanosheets possess several advantages,such as a large surface,outstanding biocompatibility,and straightforward chemical modification capability.They also have great potential as a drugcarrier.In this article,we radiolabeled GO nanosheets with99mTc,which satisfies the potential needs of microSPECT imaging probes in pre-clinical and clinical research.GO nanosheets were synthesized through the modified Hummers’method,then GO nanosheets with azide group covalently functionalized in two steps were conjugated to DOTA（1,4,7,10-tetraazacyclododecane-N,N,N,N-tetraacetic acid）and functionalized with an alkynyl group by means of click chemistry.Then through the addition and reduction of technetium-99m,the ^99mTc-DOTA-GO were attained.DOTA-conjugated GOs with lateral dimensions of 500–600 nm were synthesized.Both atomic force microscopy（AFM）and FT-IR were performed to characterize the GO-DOTA.Labeling efficiency of GO-DOTA with ^99mTc was〉90% and radiochemical purities were〉96% with purification.We successfully synthesized graphene oxide derivatives,DOTA-conjugated GOs,via Click Chemistry,and it was labeled with ^99mTc for SPECT imaging.High radiolabeling efficiency makes GO nanosheets suitable platforms for future molecular imaging research.