以啤酒废水为底物,采用空气阴极微生物燃料电池,加入50 mmol/L磷酸盐(PBS)作为缓冲溶液,考察了20℃和30℃下,MFC产电性能及生物相变化.当环境温度从30℃降低到20℃时,MFC最大输出功率从483 mW/m^2降低到435 mW/m^2,库伦效率和COD去除率变化不大.温度对阴阳两极的电位均有影响,且对阴极的影响大于对阳极的影响.以莫诺德方程对实验数据进行拟合,得到半饱和速率常数Ks分别为228 mg/L(30℃)和293 mg/L(20℃).变性梯度凝胶电泳(DGGE)图谱表明,温度变化不仅影响了阳极的优势菌群,而且对阴极微生物种类具有很大的影响.
The effects of temperature on performance and biological community structure were investigated in air-cathode microbial fuel cells (MFCs) using beer wastewater amended with 50 mmol/L phosphate buffer solution (PBS). The maximum power density decreased from 483 mW/m^2 to 435 mW/m^2 when the temperature varied from 30℃ to 20℃, meanwhile just a little decreasing on coulombic efficiency and the COD removal rate were observed. Decreasing of temperature resulted in effects both on cathode potential and anode potential, but cathode potential behaved much more sensitive to temperature. The half-saturation constants ( Ks ) obtained from the fit of Monod-type equation were 228 mg/L (30℃) and 293 mg/L (20℃) respectively. Denaturing gradient gel electrophoresis (DGGE) analysis indicated that operating temperature not only affected the predominant population of the anodic bacterial community, but also had a great impact on the diversity of the cathodic microbial population.