血脑屏障是由脑微血管内皮细胞、星形胶质细胞和周皮细胞等组成的动态生理结构,在维持中枢系统内环境稳态中发挥着重要作用。体外实验其高效、灵敏、快速、稳定等特点使其成为候选药物高通量筛选及生理病理机制研究的首选方案。该文主要对体外血脑屏障模型的种类、组成及应用进行评述,结合国内外文献报道,记述了经典模型的同时,详细描述了大量国内外的最新模型及先进技术,为中枢神经系统药物筛选及体外血脑屏障机制研究提供参考。
Blood brain barrier(BBB) is a dynamic physiological structure,which is composed of brain microvascular endothelial cells,astrocytes and pericytes,playing an important role in maintaining homeostasis in the central nervous system.In vitro experiment is the preferred alternative to the study of early central nervous system drugs screening,pathological and physiological mechanism because of high-throughput,efficient,sensitive,rapid,and accurate characteristics. This paper focuses on the types,compositions and application ofin vitro models of BBB,reviews the domestic and foreign literatures,describes the classical models and introduces the latest advanced technology,provides a reference for central nervous system drugs screening andin vitro BBB mechanism research.