中文摘要：

应用模式生物-秀丽隐杆线虫研究金属纳米镍的生殖发育毒性。采用20 nm和90 nm镍分别以2.5和5.0 μg·cm-2两个剂量对秀丽隐杆线虫进行染毒，并以微米镍和生理盐水作对照，采用后代数目、世代时间、形态和体长、寿命和半数致死率等生殖发育相关评价指标，对纳米镍生殖发育毒性进行评价。结果发现，与生理盐水对照组和微米镍组比较，秀丽线虫暴露于20 nm和90 nm镍的两个剂量组后，均表现出生殖和发育的异常（P〈0.01），并有剂量依赖性。且90 nm镍暴露对秀丽线虫后代数目、世代时间、形态和体长、寿命和半数致死天数等指标的缺陷作用均显著于20 nm镍。研究表明，纳米镍可影响秀丽线虫的生殖和发育功能，这一效应与纳米粒径和暴露浓度有关。此结论可为制定纳米镍的接触限值标准提供参考。

英文摘要：

The aim of this study is to investigate reproductive and developmental toxicity of the metallic nickel nanoparticles using Caenorhabditis elegan （C. elegans ）. First, C. elegans were treated with 20 nm or 90 nm nickel nanoparticles at the doses of 2.5 μg·cm-2 and 5.0 μg·cm-2, with 9 g·L-1 NaCl treatment and 3 μm nickel microparticle treatment as control groups. Then, the toxicity was detected by using a battery of bio-tests, including brood size, generation time, body size, life span and days of 50% lethality of C. elegans . Results indicated that, compared to control and microparticle group, abnormities in reproduction and development were induced when C. elegans were exposed to 2.5 μg·cm-2 and 5.0 μg·cm-2 of 20 nm and 90 nm nickel nanoparticles （P〈0.01）, and dose-dependent manners were observed. These results indicated that, significant toxic effects on development and reproduction were observed in C. elegans after exposure to nickel nanoparticles, which can provide a reference for determining the exposure limit standard for nickel nanoparticles.