中文摘要：

为了解决蛋白与药物分子的荧光光谱相互作用分析存在的缺点，采用小分子为荧光检测对象，以血清白蛋白（sA）为猝灭剂，研究了模拟生理条件（pH=7．4）下伯氨喹、十二烷基苯磺酸钠和西酞普兰与sA的相互作用。测得伯氨喹、十二烷基苯磺酸钠和西酞普兰与SA的结合常数Kb分别为8．16×10^4L／mol、4．14×10^6L／mol和6．08×10^7L／mol。这种改进荧光光谱法能更准确和全面地表达出sA与药物的相互作用信息。通过紫外吸收光谱相互作用分析法对改进方法进行了验证。并推测出药物与蛋白之间存在一种“点对面”的作用方式。

英文摘要：

To resolve the deficiencies in the interaction analysis by classical fluorescence spectroscopy between serum albumin （SA） and drug, a modified fluorescence spectroscopy has been applied to study the binding constant （Kh） of the interaction between SA and primaquine, sodium dodecyl benzene sulfonate and citalopram under simulated physiological conditions （pH = 7.4） , in which the fluorescence detective object is small molecule drugs and the quencher is SA. The values of Kb obtained were 8.16 × 10^4 L/mol, 4.14 × 10^6 L/mol and 6.08 × 10^7 L/mol, respectively. The rationality and correctness in the modified method has been proved based on Ultra-Violet absorption spectroscopy. The results show that this modified method can reveal the more and true information of some interactions. Thus the interaction mode between drugs and SA included point-to-area has been drawn.