中文摘要：

目的建立一种测定药物脂水分配系数的微乳液电动色谱（MEEKC）新方法，以避免测定时必须使用微乳相标记物，以及减小和评价测量误差。方法以系列化合物在MEEKC中的迁移时间tm值与其脂水分配系数之间的关系，进行非线性拟合得出微乳相的虚拟迁移时间tme，再建立标准曲线以测定所选取药物的脂水分配系数；从理论上评价测定结果准确度与tm相关性，并以此评价测定结果的准确度。结果所选取的4种药物脂水分配系数的MEEKC测定值与摇瓶法测定值基本吻合，其平均差值为0．15，其测定准确度与tm的相关性符合理论模型的分析。结论无微乳相标记物的MEEKC新方法简单、快速、可靠、重现性好，测定脂水分配系数的准确度较高．可用作药物脂水分配系数的测定。

英文摘要：

Aim To establish a novel microemulsion electrokinetic chromatography （MEEKC） method for measuring lipid-water partition coefficients （logPow） of pharmaceuticals without using microemulsion phase marker in order to avoid the error from tracing the migration time of microemulsion phase. Methods The migration time of microemulsion phase （tme） was obtained by non-linearity fitting with logPow values from literature and measured migration time （tm） of a series of organic compounds, a calibration curve for estimating logPow of pharmaceuticals was thus obtained. In addition, the accuracy of the values measured by MEEKC was evaluated. Results The logP values of 4 pharmaceuticals measured by MEEKC method presented in this paper were close to those determined by shake-flask method, and the average error between values from two methods was 0. 15 logarithm units. Furthermore, according to the suggested theory, the measurement accuracy of logP is correlated with different tm in MEEKC. Conclusion The proposed method is simple, rapid, reproducible, and reliable with high measurement accuracy, which can be useful to estimate lipid-water partition coefficients of pharmaceuticals.