通过特征颜色反应、紫外-可见光谱、傅里叶红外光谱、高效液相色谱-质谱(high performance liquid chromatography-mass spectrometry,HPLC-MS)法对蓝莓叶多酚样品进行定性分析。结果表明:蓝莓叶多酚中含有羟基、糖基、苯环、甲氧基和含氧杂环等特征基团。蓝莓叶多酚在醇溶液中最大吸收波长分别在354 nm和748 nm处。通过HPLC-MS的保留时间和多酚单体的一、二级质谱图分析,并与标准的质谱数据和保留时间比较,得知蓝莓叶多酚中有5种多酚单体,分别为表儿茶素没食子酸酯、绿原酸、甲基-芥子酸-己糖苷、5-O-阿魏酰奎尼酸、槲皮素3-O-葡萄糖苷。
Specific chemical reactions, ultraviolet-visible spectroscopy, infrared absorption spectroscopy, and high performance liquid chromatography-mass spectrometry(HPLC-MS) analyses indicated that polyphenols from blueberry leaves contained the characteristics groups of hydroxide radical, glycosyl, benzene ring, methoxyl and oxygen heterocycle. The maximum absorption wavelengths of polyphenols from blueberry leaves were 354 nm and 748 nm in alcohol. Five individual polyphenols i.e., epigallocatechin gallate, chlorogenic acid, methyl-sinapic acid-hexoside, 5-O-feruloylquinic acid and ouercetin 3-O-glucoside were tentatively identified by comparing their mass spectral data and retention times with those of reference standards and published data.