依据JJF 1059—1999《测量不确定度评定与表示》和CNAS—GL06《化学分析中不确定度的评估指南》规定的测量不确定度的基本方法,建立数学模型,分析高效液相色谱法测定鸡肉中磺胺类药物残留的不确定度来源,并对各分量进行量化,给出5种磺胺类药物残留测定结果的扩展不确定度,评定结果表明:影响检测结果不确定度的主要因素为标准曲线拟合、样品前处理过程和测量重复性等。
A mathematical model was established according to the basic method for uncertainty evaluation from JJF 1059—1999 and CNAS-GL06.The sources of uncertainty for determining sulfonamide residues in chicken by high performance liquid chromatography(HPLC) were analyzed and each component of uncertainty was quantified.The expanded uncertainty for five sulfonamides residues results was calculated.The major uncertainty sources were standard curve fitting,sample pretreatment and measurement repeatability.