中文摘要：

目的检测临床分离铜绿假单胞菌对10种常用抗生素的敏感性及其I类整合子的分布，探讨整合子的存在与细菌耐药性的相关性。方法收集临床分离鉴定的铜绿假单胞菌58株，纸片扩散法测定其对10种常用抗生素的敏感性，PCR法检测I类整合子整合酶基因，分析I类整合子与细菌耐药性的关系。结果58株铜绿假单胞菌对10种常用抗生素的耐药率为29．31％～74．14％，I类整合子的检出率为70．69％。I类整合子阳性菌株对左氟沙星及庆大霉素表现出较高的耐药率，其耐药率明显高于I类整合子阴性菌株（x^2=12．35、5．635，P〈0．05）．结诊铜绿偶堕晌菌的耐药件与T娄罄合千密切相美．T类罄合子对铜绿偶重晌萧耐药韶着雷要作用。

英文摘要：

Objective To detect Pseudomonas aeruginosa isolated and their sensitive to 10 antibiotics that commonly used clinically and the distribution of their class I integrons and their correlation with drug resistance. Methods Fifty-eight strains of Pseudomonas aeruginosa isolated clinically were collected, their sensitive to 10-eomrnonly-used antibiotics was determined by K-B disk diffusion method, and the integrase gene of class I integron was amplified by PCR. The relationship between class I integron and the drug resistance was analyzed. Results Among the 58 strains, the resistance rate to 10 alltibiotics that usually used clinically was 29.31%-74.14%, and the detection rate of the integrase gene of class I integron was 70.69% （41/59）. The po- sitive strains of class I integron had a higher drug resistance rate than that of the negative strains, and showed a higher resistance to levofloxacin and gentamyein （Z2 = 12.35,5. 635 ; P 〈 0.05 ）. Conclusion The drug resistance of Pseudomonas aeruginosa is closely associated with class I integron that plays an important role of drug resistance of the bacteria.