中文摘要：

[目的]研究邻苯二甲酸二丁酯（ DBP）宫内暴露对亲代孕鼠及F1代子鼠生殖毒性的影响。[方法]染毒组通过灌胃方式给母鼠染毒，染毒剂量分别为20 mg／（kg· d）、100 mg／（kg· d）和500 mg／（kg· d）。用酶联免疫法检测血清中雌二醇（ E2）、黄体生成素（ LH ）、促卵泡刺激素（ FSH ）及睾酮（ T ）的水平。[结果]本实验剂量的DBP对母鼠各脏器无明显毒作用，但对血清中性激素水平影响显著，与溶剂对照组相比，中、高剂量组（100、500 mg／（kg· d））母鼠的T水平均明显下降（P＜0．05），而高剂量组的母鼠血清中FSH含量增加（P＜0．001）；随着DBP染毒剂量增加，母鼠妊娠期体重增长明显下降（P＜0．05）；雌性子鼠血清中性激素水平测定结果显示：分娩后第7天（PND7），雌性子鼠的 LH、T 水平随 DBP 染毒剂量增加而下降，各处理组间均存在差异（ P ＜0．05）；至第26天（PND26），雌性子鼠的E2水平随染毒剂量的增加下降明显，各组间差异显著（P＜0．05），中、高剂量组的雌性子鼠FSH水平低于对照组（P＜0．05），高剂量组的T水平明显升高（P＜0．05）。[结论]母鼠妊娠后期DBP暴露对子代有明显的毒性效应，较低剂量的DBP也可改变子代血清中性激素水平。

英文摘要：

[Objective ] To observe the effects of intrauterine DBP exposure on the reproductive system of both mother rats and mature F 1 generation rats . [ Methods ] The pregnant rats were administered DBP in various doses （20 mg/kg,100 mg/kg and 500 mg/kg） by gavage.Enzyme linked immunosorbent assay （ ELISA ） was applied to detect the hormone levels in serum , including estrogen two alcohol （ E2 ） , luteinizing hormone （ LH ） , follicle stimulating hormone （ FSH ） and testosterone （ T ） . [ Results] The results showed that the experimental doses of DBP exerted significant effect on serum hormone levels but no significant effects on various organs of mother rats .Compared with the control group , serum T levels in the groups treated with 100 mg/kg and 500 mg/kg were significantly decreased （P〈0.05）,while FSH level increased in 500 mg/kg dose group （P〈0.05）;with the increase of DBP exposure doses, the weight of mother rats during gestational period decreased significantly （P〈0.05）.For the F1 female rats, on PND7, the serum LH and T levels decreased with DBP exposure doses increased （P〈0.05）;on PND26, the E2 levels of the female pups decreased significantly （P〈0.05）,the FSH levels of rats in dosed group （100 and 500 mg/kg） were significantly lower than those in the control group （P〈0.05）,and T level in 500 mg/kg DBP dosed group increased （P〈0.05） obviously. [Conclusion] Intrauterine DBP exposure exerts significant toxic effect on the reproductive system of F 1 rats; even low dose of DBP can lead to changes in the serum hormone levels , which can be illustrated by the various metabolic profiles.