本文评述了自1999年以来近红外荧光探针和标记试剂及其在生物分析中的应用进展。包括:菁染料、噻嗪和唣嗪染料、含四吡咯基团染料(酞菁和卟啉)、罗丹明类、BODIPY、稀土离子配合物和量子点等。描述了它们在荧光测定和毛细管分离荧光检测以及免疫荧光分析方面的应用。引用文献75篇。
The near-infrared fluorescent probe and its application in biological analysis are reviewed. The indodicarbocyanines, tetrapyrrrole dyes (phthalocyanine and porphyrine), oxazine and thiazine dyes, rhodamine dyes(rhodamine 800,Alexa dyes etc), BODIPY, lanthanide probes, quantum dots, etc. are introduced, and their biological and analytical applications are described.