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中文摘要：
蛋白质可逆磷酸化过程几乎调节着生命活动的所有过程，且与某些重大疾病的发生发展密切相关。因此，对磷酸化蛋白的测定具有极为重要的意义。本项目拟设计合成具有下述结构特征的新型荧光探针以对磷酸基有选择性反应的咪唑基为反应基团，以长波长的荧光量子点和花菁类、二吡咯甲烷-二氟化硼类化合物为荧光团。在此基础上，将探针对磷酸化蛋白进行选择性标记，使其适于毛细管电泳-激光诱导荧光检测和细胞成像，建立简便、灵敏、无损的磷酸化蛋白检测方法，并用于细胞和蛋白质复合物中磷酸化蛋白的分析、蛋白激酶和磷酸酶活性的研究。本项目的完成将促进基于荧光探针的毛细管电泳和细胞成像法的发展，丰富蛋白质组学的研究方法，加深对与蛋白质磷酸化相关的生理及病理过程的了解；为从细胞和分子水平上深刻认识生命的化学本质与基本过程提供重要的手段，同时也将为重大疾病的早期诊断及疗效评价提供有效的工具。
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期刊论文
Rapid and sensitive determination of phosphoamino acids in phosvitin by N-hydroxysuccinimidyl fluore
6-Oxy-(acetyl ethylenediamine) fluorescein, a novel fluorescent derivatization reagent for carboxyli
Real-time imaging of nitric oxide production in living cells with 1,3,5,7-tetramethyl-2,6-dicarbetho
Determination of primary biogenic amines in various food products using isocratic HPLC with 3-(4-chl
Ultra-trace level determination of nitrite in human saliva by spectrofluorimetry using 1,3,5,7-tetra
Sensitive determination of aliphatic amines by high-performance liquid chromatography with a new flu
Determination of biogenic amines in apples and wine with 8-phenyl-(4-oxy-acetic acid N-hydroxysuccin
8-Phenyl-(4-oxy-acetic acid N-hydroxysuccinimidyl ester)-4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,
Determination of phosphoamino acids by 6-oxy-(N-succinimidyl acetate)-9-(2 '-methoxycarbonyl)fluores
Sensitive determination of ultra-trace nitric oxide in blood using derivatization-polymer monolith m
Investigation of the effect of tanshinone IIA on nitric oxide production in human vascular endotheli
Determination of nitric oxide in hydrophytes using poly(methacrylic acid-ethylene glycol dimethacryl
Sensitive determination of nitric oxide in some rat tissues using polymer monolith microextraction c
Quantification of biogenic amines in human plasma based on the derivatization with N-hydroxy-succini
Quaternary ammonium chitosan derivative dynamic coating for the separation of veterinary sulfonamide
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