中文摘要：

目的研究宫颈癌细胞中人粒细胞巨噬细胞集落刺激因子（GM～CSF）的表达对环氧合酶-2（COX-2）和诱导性一氧化氮合酶（iNOS）表达的影响，探讨GM—CSF在宫颈癌发生发展中的作用。方法收集临床宫颈癌标本52例及正常宫颈15例和宫颈上皮内瘤变（CIN）组织标本21例作为对照进行免疫组织化学染色，分析宫颈癌组织GM—CSF的表达与COX-2和iNOS表达的相关性；为了从细胞水平进一步论证其相关性，本实验使用宫颈癌细胞系Caski细胞，Caski细胞经GM—CSF转染后过表达GM—CSF，并通过RT—qPCR和Westernblot检测COX-2和iNOSmRNA及蛋白的表达情况，以空质粒转染的Caski细胞为对照。在细胞模型中探讨GM—CSF强制表达对COX-2和iNOS表达的影响。同时，通过GM—CSFsiRNA干扰Caski细胞GM—CSF表达后，用Ⅳr_qPCR及Westernblot方法检测iNOS／COX-2mRNA及蛋白表达情况。结果免疫组化结果显示，宫颈癌组织GM—CSF表达明显低于正常组和CIN组（P均〈0．05），而COX-2及iNOS的表达显著高于正常组（P〈0．01），但与CIN组无明显差异；统计学分析发现GM—CSF与COX-2／iNOS的表达呈负相关（r=-0．542／-0．499）。细胞水平结果显示与空质粒转染的细胞相比．转染GM—CSF的Caski细胞COX-2／iNOS在mRNA及蛋白表达水平都有显著下降（P均〈0．05）；GM—CSF在被干扰后，干扰组与对照组相比COX-2／iNOS在mRNA及蛋白表达水平有明显差异（P均〈0．05）。结论在组织和细胞表达水平GM—CSF与COX-2／iNOS的表达均呈负相关，GM—CSF可能通过下调COX-2／iNOS表达而具有抗宫颈癌作用。

英文摘要：

This study performed to explore the roles of GM-CSF in the occurrence and development of cervical cancer by evaluating the effect of GM-CSF expression on COX-2 and iNOS in cervical cancer cells. Firstly, Immunohistochemieal （IHC） assay was used to detect GM-CSF, iNOS and COX-2 in 52 cervical cancer, 21 cervical intraepithelial neoplasia （CIN） and 15 normal cervical samples, for analyzing the correlation between GM- CSF and COX-2/iNOS. To explore whether GM-CSF over-expression had an effect on COX-2/iNOS in cellular models, Caski cells were introduced and transfected with GM-CSF to improve the expression of GM-CSF, furthermore, the mRAN and protein expression of COX-2 and iNOS were detected with RT-qPCR and Western blot, respectively. In control trial, the expression of GM-CSF was silenced in Caski cells to compare the mRNA and protein expression of COX-2/iNOS. IHC results showed GM-CSF in carcinoma group was decreased （P 〈 0.05） as compared with normal tissues and CIN groups, but the COX-2 and iNOS expression were opposite. And the statistic analysis found that the expression of GM-CSF had a negative correlation with COX-2/iNOS（r=-0.542/-0.499）; mRAN expression and protein expression of COX-2 and iNOS were decreased in the cells transfected with GM- CSF（P〈 0.05）, and they were increased when GM-CSF was silenced. The results indicated that GM-CSF may play an anti-cervical carcinoma role by decreasing the expression of COX-2/iNOS.