中文摘要：

为筛选兔瘟病毒在兔肾上皮细胞（rabbit kidney epithelial cells，RK13）上的受体，从RK13细胞中提取基因组总RNA，然后用链霉亲和素磁珠（SA—PMP）纯化mRNA，再用SMART（Switching methanism at 5′end of RNA transcript）技术合成cDNA第1链。采用长距离PCR（long-distance—PCR，LD—PCR）方法扩增双链cDNA，经纯化后克隆到真核表达载体pEXP—Lb中。最后，电转化宿主菌（DH5α），构建了RK13细胞的全长cDNA真核表达文库。鉴定结果表明。该文库插入的cDNA片段平均长度达到1．0kb．库容量达到2．55×10^5CFU．

英文摘要：

The full length cDNA library of rabbit kidney epithelial （RK-13） cells was constructed to identify RHDV receptors. Total mRNAs were isolated and purified from RK-13 cell and then the first strand cDNAs were synthesized by reverse transcription （RT） with RT primer （CDSIII） and the double-stranded cDNAs were amplified by long-distance-PCR. Subsequently, the ds cDNAs were inserted into the eukaryotic expression vector （pEXP-Lib） and transformed into E. coli cells （DH5ct）. The results showed that the cDNA library contained 2.55 × 10^5 clone with averaged inserts about 1.0 Kb.