中文摘要：

α1-肾上腺素受体（α1-Adrenergic receptor，α1-AR）是G蛋白偶联受体（G-protein coupled receptor，GPCR），也是内源性儿茶酚胺、去甲肾上腺素和肾上腺素最重要的靶受体之一．α1-R广泛分布于机体印争种器官、组织和细胞中，并介导多种生理效应，如血管收缩、蛋白质合成及心脏变力变时作用等。很多研究已经证实，α1-AR及其信号转导通路与许多心血管疾病存在密切关系．

英文摘要：

In order to find candidate proteins that are potentially associated with the phenotype in neonatal rat cardiomyocytes treated with phenylephrine（PE） , the differential proteins expression pattern after α-adrenergic receptor stimulation in neonatal cardiomyocytes was analyzed by using a series of methods, including two dimensional polyacrylamide gel electrophoresis, PDQuest 2-DE software analysis and peptide mass fingerprint mapping based on MALDI-TOF-MS. The good two-dimensional pattern with a high resolution and reproducibility was obtained. Totally 14 differential protein spots were found. Out of these, 11 proteins were preliminarily identified. Desmin, Txndc5, etc. were found to be up-regulated in PE-treated cardiomyocytes, while caspase-11,4-hydroxyphenylpyruvic acid dioxygenase, etc. were found to be down-regulated. It suggests that the differential expression analysis of proteome may be useful to the further study of the related proteins and the molecular markers of cardiovascular diseases.