中文摘要：

目的探讨胚胎干细胞-D3株（ES-D3）联合碱性成纤维细胞生长因子（bFGF）移植治疗大鼠急性心肌梗死,是否有利于心脏结构的恢复和心功能的改善。方法Wistar大鼠40只随机均分成5组,分别为正常对照组（组1）、梗死未治疗组（组2）、培养基注射组（组3）、ES-D3移植组（组4）、ES-D3＋bFGF移植组（组5）。大鼠急性心肌梗死造模后1周移植体外分化并经标记的ES-D3,4周后进行心功能及组织学检测。结果ES-D3体外能分化为心肌样细胞。梗死后4周检测表明,移植细胞在大鼠体内稳定存活。心功能及组织学检测表明,组2与组3大鼠无显著差异（P〉0.05）。与组2比较,组4和组5大鼠心肌梗死面积均显著减小,左心室重量减轻（P〈0.01）;毛细血管密度显著增高（P〈0.01）;左心室功能显著改善。结论急性心肌梗死后移植ES-D3可以促进大鼠心血管新生、阻止心室重构、减少瘢痕面积、显著改善心功能,联合应用bFGF可进一步获益。

英文摘要：

Objective To investigate the feasibility of recovering the cardiac structure and improving the cardiac function after the transplantation of embryonic stem cells-D3 （ES-D3） combined with basic fibroblast growth factor （b-FGF） into the acute myocardial infarction （AMI） . Methods Forty Wistar rats were used and randomly divided into 5 groups equally, including the normal control group （group 1）, the myocardial infarction without treatment group （group 2）, the medium injected group （group 3）, the ES-D3 transplanted group （group 4）, the combination of ES-D3 and bFGF transplanted group （group 5）. One week after the acute myocardial infarction （AMI）, rats were injected with the in vitro differentiated ES-D3 labeled by BrdU. Then the cardiac function and histology were measured in week 4 after the transplantation. Results ES-D3 could differentiate into cardiomyocytes in vitro, and could survive steadily for 4 weeks in vivo after the transplantation into AMI＇s model. There were no significant difference between group 2 and group 3 in cardiac function（P〉0.05）. Compared with group 2, the infarction size and left ventricular weight（LVW） were reduced significantly（P〈0.01）in group 4 and group 5, and capillary density was increased significantly（P〈0.01）. The functions of the left ventricle were remarkably improved. Conclusion The transplantation of ES-D3 can promote angiogenesis, improve the cardiac function, prevent the remodeling process of the left ventricle and reduce the infarct size, and further benefits can be obtained by co-administration of bFGF.