中文摘要：

目的 研究p38丝裂原活化蛋白激酶（MAPK）在血小板源性生长因子（PDGF）诱导的细胞迁移中的作用。方法 PDGF刺激小鼠NIH3T3细胞后，利用免疫印迹法检测p38磷酸化程度的改变情况。利用Transwell细胞迁移系统来观察PDGF对NIH3T3细胞迁移的诱导作用，以及p38基因敲除对PDGF诱导的细胞迁移的影响。结果 PDGF能显著诱导NIH3T3细胞迁移（P〈0．001），且在此过程中p38能被磷酸化激活，p38基因敲除能阻断PDGF诱导的细胞迁移（P〈0．001）。结论 p38 MAPK参与了PDGF诱导的细胞迁移的调控。

英文摘要：

Objective To investigate the role of p38 rnitogen-activated protein kinase （MAPK） in the cell migration induced by platelet-derived growth factor （PDGF）. Methods The phosphorylation degree of p38 in NIH 3T3 cell line, which was stimulated with PDGF, was analyzed with Western blot. The effects of PDGF treatment on the induction of the migration of NIH 3T3 cells, as well as p38 gene knockout on the PDGF-induced cell migration, were observed by using Transwell cell migration system Results The migration of NIH 3T3 cells was significantly induced by PDGF treatment （P〈0. 001）, and in this process, p38 was activated by phosphorylation. Furthermore, the cell migration induced by PDGF was inhibited by p38 gene knockout （P〈0. 001）, as showed in the experiment performed with 1938 gene knockout mouse embryonic fibroblasts. Conclusion p38 MAPK plays an important role in the regulation of cell migration induced by PDGF.