中文摘要：

目的：构建可受Tet-on和Cre/loxP系统双调控的HCV NS5B真核表达载体,为建立可严格调控HCVNS5B蛋白表达的转基因小鼠奠定基础。方法：以真核表达载体pBI-3为载体构建骨架,在其启动子下游依次插入luc报告基因、BGHpA和NS5B基因片段,并分别在luc报告基因上游和BGHpA尾下游引入一个loxP位点。结果：成功构建了可受Tet-on和Cre/loxP系统双调控的HCV NS5B真核表达载体pBI-3/luc-BGHpA-NS5B。结论：pBI-3/luc-BGHpA-NS5B真核表达载体的成功构建为可严格调控HCV NS5B蛋白表达转基因小鼠的建立打下了良好的基础。

英文摘要：

Objective： To construct a HCV NS5B eukaryotic expression vector dually-controlled by Tet-on and Cre/loxP for producing transgenic mice which can strictly regulate expression of HCV NS5B. Methods： By utilizing the eukaryotic expression vector pBI-3 as vector framework, the luc reporting, the BGH pA tail and the NS5B fragment were orderly inserted in the downstream of the promoter, and a loxP site was introduced respectively in the upstream of the luc reporting and downstream of the BGH pA tail. Results： The HCV NS5B eukaryotic expression vector pBI-3/ luc-BGH pA-NS5B, which could be dually-controlled by Tet-on and Cre/loxP, were successfully constructed. Conclusion： The establish of the pBI-3/ luc-BGH pA-NS5B provides the basis for the establishment of transgenic mice that could strictly regulate the expression of HCV NS5B.