中文摘要：

目的探讨铜蓝蛋白（Cp）在石英诱导的人胚肺成纤维细胞（HELF）组蛋白修饰改变中的作用以及人第十号染色体缺失的磷酸酶及张力蛋白同源的基因（PTEN）在其中的影响。方法不同浓度的石英（50、100、200μg／m1）和不同浓度的Cp（10、20、30μg／m1）作用HELF细胞24h，用蛋白免疫印迹（WesternBlot）实验检测蛋白质赖氨酸乙酰化（acety—lys）水平以及组蛋白H2B、H3、H4乙酰化和H3甲基化水平。200μg／ml石英作用于HELF细胞和转染PTENshRNA的HELF细胞（PT）1h后，加入30μg／mlCp作用24h，用Westernblot检测acetylys水平，组蛋白H2B、H3、H4乙酰化和H3甲基化水平。结果石英暴露可诱导蛋白质acety-lys水平的增高，组蛋白H2B（1ys5／12）、H3（1ys9／14）、H4（1ysl2）乙酰化水平增加，组蛋白H3的2位精氨酸的甲基化水平降低。Cp可以逆转石英暴露所致的蛋白质acetylys水平的增加，组蛋白H3、H4赖氨酸乙酰化水平的增加和组蛋白H3甲基化水平的减少。抑制PTEN的水平后，观察不到Cp对上述现象的逆转。结论Cp可以逆转石英暴露诱导的组蛋白乙酰化和甲基化改变，PTEN参与了Cp对石英诱导的组蛋白修饰作用的改变。

英文摘要：

Objective To explore the roles of ceruloplasmin （Cp） in histone modifications in human embryonic lung fibroblasts （HELFs） induced silica and the effects of phosphatase and tension homolog deleted on chromosome ten（PTEN） in this process. Methods HELFs were exposed to different concentrations of silica （50, 100, 200 μg/ ml） or Cp （10, 20, 30μg/ml） for 24 h, and the level of protein lysine acetylation, of histone H2,H3 and H4 acetylation and histone H3 methylation were checked by werstern blot assay. HELFs and cells transfected with PTEN shRNA （PT） were treated with 200μg/ml silica for 1 h, then added 30 μg/ml Cp for 24 h. Acetylation levels of protein lysine, historic. H2, H3, H4 and methylation level of histone H3 were detected by werstern blot assay. Results Silica could induc the high level of protein lysine acetylation and acetyl-histone H2B （lys5/12）, acetyl-histone H3 （ lys9/14）, acetyl- histone H4 （lysl2） and the low level of methyl-histone （arg2） , which could be reversed by Cp, in no exception for acetyl-histone H2B （lys5/12）. Cp couldn＇t reverse histone modifications induced by silica when inhibited PTEN. Conclusion Cp could reverse silica-induced the change of histone acetylation and histone methylation, and PTEN involved in this process.