中文摘要：

目的探讨母体孕期应激与其子代痉挛症发生的相关性。方法痉挛模型的建立和放射性配基结合实验：受孕母鼠11只,随机分为两组,对照组5只,应激组6只。应激组进行慢性刺激（强迫母鼠在4℃冷水中游泳,1次/d,5min/次）直至分娩;对照组母鼠孕期不予刺激。仔鼠生后第12天腹腔注射N-甲基-D-天冬氨酸（N-Methyl-D-Aspartic acid,NMDA,7mg/kg）诱发痫样发作,通过[3H]MK-801结合NMDA受体,[3H]muscimol结合GABAA受体,从液闪仪中读数,计算受体含量,比较仔鼠皮层中NMDA受体含量。结果应激组仔鼠大脑皮层内兴奋性NMDA受体含量平均值为10 537fmol/L;正常组受体含量平均值为4 927fmol/L,差异有统计学意义（P=0.025）。结论母孕期应激与子代痉挛症发病具有相关性,能加大子代痉挛患病几率。

英文摘要：

Objective To study the correlation between gestational stress and pathogenesis of spasms in infantile rats.Methods A rat spasm mode was established to perform radioligand binding assay.Eleven pregnant rats were randomly divided into control group（n=5） and stress group（n=6）.Rats in stress group were chronically stimulated until they gave birth（the pregnant rats were forced to swim in water at 4℃ for 5 minutes,once a day）.Rats in control group were not stimulated.Twelve days after they gave birth,the rats were intra-abdominally injected with N-methyl-d-aspartic acid（7mg/kg） to induce epilepsy.Levels of[ 3H]MK-801 binding NMDA receptor and[ 3H]muscimol binding GABAA receptor were measured with a stroscope.Different N-methyl-d-aspartic acid receptor levels in cortex of infantile rats were measured and compared.Results The excitable N-methyl-d-aspartic acid receptor level was higher in cortex of infantile rats of stress group than in that of those of control group.Conclusion Stress of gestational rats is correlated with the pathogenesis of spasm in infantile rats,and can increase the incidence of spasm in the offspring.