中文摘要：

目的探讨Akt3基因敲除对坐骨神经结扎小鼠神经病理性痛行为学的影响。方法C57BL／6小鼠分为Akt3基因敲除组（Akt3-／-，n=12）和野生组（WT，n=12）。随机编号后，在小鼠右侧制作坐骨神经慢性挤压（CCI）模型。观察术前1d，术后1，3，5，7，10，14，17，21d小鼠机械缩足阈值（Paw withdrawal mechanical threshold，PWMT）和热缩足潜伏期（Paw withdrawal thermal latency，PWTL）。结果两组小鼠基础PWMT[右侧：（1．09±0．20）g，（1．17±0．22）g；左侧：（1．17±0．15）g，（1．22±0．23）g，P〉0．05]和PWTL[右侧：（6．18±1．11）S，（6．20±1．25）S；左侧：（5．82±0．91）S，（5．92±1．71）S，P〉0．05]差异无统计学意义。术后1d，与基础值相比，Akt3-／-组和WT组小鼠右侧足PWMT和PWTL明显降低（P〈0．05），并持续到术后第21天。Akt3-／-组小鼠右侧足PWMT[第3天：（0．42±0．22）g，（0．72±0．36）g；第17天：（0．29±0．15）g，（0．49±0．19）g；第21天：（0．27±0．18）g，（0．56±0．15）g，P〈0．05]和PWTL[第5天：（2．43±0．68）s，（3．13±0．52）S；第17天：（2．43±1．26）S，（3．84±1．29）S；第21天：（2．14±1．23）s，（4．07±1．26）S，P〈0．05]明显低于WT组。Akt3-／-组左侧足PWMT和PWTL与WT组小鼠相比差异无统计学意义（P〉0．05）。但是与左侧足相比，Akt3-／-组和WT组小鼠右侧足PWMT和PWTL均明显降低（P〈0．05）。结论Akt3基因敲除后，小鼠坐骨神经结扎诱发的神经病理性疼痛加重。

英文摘要：

Objective To investigate the effects of Akt3 gene knockout on neuropathic pain behaviors induced by chronic constriction injury , of sciatic nerve （CCI）. Methods Experiment was divided into two groups ： Akt3 knockout group（ Akt3-/-, n = 12 ）, wild type group（ WT, n = 12 ）. Randomly numbered, the right sciatic nerve of mice were received the operation of chronic constriction injury. Paw withdrawal mechanical threshold （PWMT） and paw withdrawal thermal latency （PWTL） were tested on day 1 before operation and day 1,3,5,7,10,14,17, 21 afterCCI. Results The basic values of PWMT（right：（1.09±0.20）g,（1.17±0.22）g;left：（1.17±0.15） g,（1.22±0.23）g,P〉0.05） andPWTL（right：（6.18±1.11）s,（6.20±1.25）s;left：（5.82±0.91）s,（5.92 ± 1.71 ） s, P 〉 0.05 ） had no statistically significant differences between two groups. On day 1 after operation, compared with basic values ,the PWMT and PWTL of the right paw in both Akt3-/- group and WT group decreased significantly （P 〈 0.05 ）, and at least lasted up to day 21. The PWMT（ 3d ： （ 0.42 ± 0.22 ） g, （0.72 ± 0.36） g; 17d： （0.29 ±0.15）g,（0.49 ±0.19）g;21d： （0.27 ±0.18）g, （0.56 ±0.15）g, P〈0.05） and PWTL（Sd： （2.43 ± 0.68）s,（3.13 ±0.52）s;17d：（2.43 ±1.26）s,（3.84 ± 1.29）s ;21d：（2.14 ±1.23）s,（4.07 ±1.26）s, P〈 0.05 ） of the right paw in Akt3-/- group was significantly lower than those in WT group. The PWMT and PWTL of the left paw in Akt3-/- group and WT group had no obvious differences （P 〉 0.05 ）. However, compared to left paw, the PWMT and PWTL of the right paw of the two groups were obviously lower （P 〈 0.05 ）. Conclusion The neuropathie pain induced by CCI increased in Akt3 gene knockout mice.