采用微波预处理菘蓝(Isatis indigotica Fort)种子,待种子出土萌发后进行UV-B辐射(10.08kJ m^2d^-1,PAR=220μmol m^-2d^-1)处理6d,研究了经不同时间微波辐照后菘蓝种子发育而成的幼苗在UV-B辐射下非酶类抗氧化剂(紫外吸收物质、花青素甙、谷胱甘肽、抗坏血酸和脯氨酸)的生物合成情况.结果显示,在UV-B辐射条件下,4种微波预处理均能不同程度促进紫外吸收物质、化青素甙、谷胱甘肽、抗坏血酸和脯氨酸的生物合成,降低丙二醛含量,增强幼苗对UV-B伤害的抗性.这表明,适当时间的微波预处理可以提高植物对增强UV-B辐射的抵抗能力,与此同时,过长时间微波处理也会抑制植物的生理生化代谢和非酶类抗氧化剂的生物合成,加重对植物的伤害.因此,微波具有相悖的防护效应,存生物学上应用时其剂量的大小十分关键.表1参17
The effects of different time lengths of microwave radiation on biosynthesis of non-enzymatic antioxidants in Isatis indigotica seedlings with enhanced UV-B damages were studied. The seeds soaked with water for 3 h were exposed to microwave, and then subjected to enhanced UV-B radiation (10.08 kJ m^-2 d^-1, PAR=220 /amol m^-2 d ^-1) for 6 d after the seeds germination. The results showed that four treatments with different time improved the resistant capability to UV-B radiation by increasing the concentration of GSH, UV absorbance, anthocyanin, AsA and proline, and decreasing the concentration of MDA and GSSG. Microwave radiation of suitable doses could stimulate the synthesis of non-enzymatic antioxidants and improve the resistant capability to UV-B radiation. However, bad effects were also caused by microwave radiation. Therefore, the dose to be selected for applying in agriculture is the key. Tab 1, Ref 17