中文摘要：

目的研究蕨类植物中4CL基因的表达与分析,为蕨类植物次级代谢产物的积累和调控奠定理论基础。方法运用CHIPS、CUSP和Codon W在线程序对自主克隆的香鳞毛蕨4CL基因（Df4CL）序列进行分析,并与大肠杆菌、酵母菌、拟南芥、烟草等基因进行比较,对了解Df4CL基因密码子使用特性,为其选择合适的表达系统具有重要意义。结果 Df4CL基因对ATGC选择没有偏向性,并且与小立碗藓的密码子使用偏好性相一致。在密码子使用频率上,Df4CL基因与拟南芥的差异小于与烟草、大肠杆菌、酵母的差异;基于4CL基因的密码子使用偏性的系统聚类分析表明,香鳞毛蕨与小立碗藓、拟南芥聚为一类。结论预示Df4CL基因更适合在拟南芥中外源表达。

英文摘要：

Objective Expression and analysis of 4CL gene of ferns, which lays the theoretical foundation for the accumulation and regulation of secondary metabolites of ferns. Methods Cloned 4CL gene（Df4CL） sequence from Dryopteris fragrans was analyzed by CHIPS, CUSP, and Codon W programs online, and then compared with Escherichia coli, yeast, Arabidopsis thaliana, and Nicotiana tabacum L. It is important to identify the codon usage of Df4 CL gene and select appropriate expression systems. Results Df4 CL gene had not the bias toward the synonymous codons with ATGC at the third codon position, but had the preference consistency with Physcomitrella patens codon usage. The differences in codon usage frequency between Df4 CL gene and A. thaliana were less than those in N. tobacum, E. coli, and yeast. Based on 4CL gene codon usage bias of cluster analysis, it was shown that D. fragrans, P. patens, and A. thaliana were classed into one group. Conclusion The A. thaliana expression system may be more suitable for the exogenous expression of Df4 CL gene of D. fragrans.