东篱科研大数据发现系统（DRDS）

位置：成果数据库 > 期刊 > 期刊详情页

MiR-125a-5p is Upregulated in Plasma of Residents from An Electronic Waste Recycling Site

ISSN号：1673-5897
期刊名称：《生态毒理学报》
时间：0
分类：X171.5[环境科学与工程—环境科学]
作者机构：[1]State Key Joint Laboratory for Environmental Simulation and Pollution Control, College of Environmental Sciences and Engineeringand Center for Environment and Health, Peking University, Beijing 100871, P. R. China, [2]Basic Medical College, Tianjin Medical University, Tianjin 300070, P. R. China
相关基金：National Natural Science Foundation of China（21322705,41121004,21190051,and 21177091）

作者： Li Ran, Qiu Xinghua, Yang Qiaoyun, Li Keqiu, Li Guang

关键词： E-WASTE, originated, POLLUTANTS, Micro, RNA, mi, R-125a-5p, EPIGENETICS, OXIDATIVE, Stress, e-waste originated pollutants, micro RNA, mi R-125a-5p, epigenetics, oxidative stress

中文摘要：

The mechanism of health effects caused by organohalogen pollutants, e.g., toxins from electronic waste(e-waste), is poorly understood. We supposed that micro RNAs(mi RNAs), an important post-transcriptional regulator, could play a role in this process. In this study, fasting peripheral blood samples were collected from residents living at an e-waste site in northern China and a nearby reference population. Concentrations of e-waste related organohalogen pollutants in plasma from the exposure group were higher than the corresponding measurement in the reference group. Correspondingly, sixty mi RNAs in plasma showed > 2-fold change between the two groups in microarray analysis. Among them, mi R-125a-5p was confirmed to be upregulated by q RT-PCR and its validated targets were enriched in responses to xenobiotics and cancer related pathways. Furthermore, significant positive correlations were found between levels of mi R-125a-5p in plasma and reactive oxygen species(ROS) in polymorphonuclear neutrophil leukocytes(P < 0.05). These evidences suggested oxidative stress might be an intermediate between e-waste related POPs exposure and alteration of plasma mi RNA.

英文摘要：

The mechanism of health effects caused by organohalogen pollutants, e.g., toxins from electronic waste（e-waste）, is poorly understood. We supposed that micro RNAs（mi RNAs）, an important post-transcriptional regulator, could play a role in this process. In this study, fasting peripheral blood samples were collected from residents living at an e-waste site in northern China and a nearby reference population. Concentrations of e-waste related organohalogen pollutants in plasma from the exposure group were higher than the corresponding measurement in the reference group. Correspondingly, sixty mi RNAs in plasma showed 2-fold change between the two groups in microarray analysis. Among them, mi R-125a-5p was confirmed to be upregulated by q RT-PCR and its validated targets were enriched in responses to xenobiotics and cancer related pathways. Furthermore, significant positive correlations were found between levels of mi R-125a-5p in plasma and reactive oxygen species（ROS） in polymorphonuclear neutrophil leukocytes（P 0.05）. These evidences suggested oxidative stress might be an intermediate between e-waste related POPs exposure and alteration of plasma mi RNA.