中文摘要：

羟基红花黄色素A（hydroxysafflor yellow A,HSYA）是从菊科植物红花中提取的查尔酮类化合物。本实验观察HSYA对低氧状态（1%O2）下人脐静脉内皮细胞株（Eahy926）增殖的促进作用及其调节Eahy 926细胞中林希氏基因（von Hippel-Lindau gene,VHL）,抑癌基因p53（tumor suppressor gene p53,p53）和降解缺氧诱导因子-1α（hypoxia-inducible factor 1α,HIF-1α）的作用。采用MTT法测定低氧状态下HSYA（100、10和1μmol.L-1）对Eahy926细胞增殖率的影响。免疫组织化学方法测定VHL与p53蛋白表达数量和定位。RT-PCR方法检测细胞中VHL、p53和HIF-1α的转录水平。Western blotting方法检测VHL、p53和HIF-1α的蛋白表达。与低氧模型对照组相比,HSYA（100μmol.L-1）促使细胞增殖率升高,HIF-1α转录和蛋白表达水平显著增强,呈时间依赖性。给药8 h后,VHL与p53的蛋白表达和转录水平均明显降低。HSYA诱导HIF-1α表达增加并提高低氧状态下细胞增殖率的作用,可能通过抑制VHL和p53两种HIF-1α的降解调节因子实现。

英文摘要：

In present study, we investigated the mechanism of regulating HIF-1α expression by hydroxysafflor yellow A （HSYA）in Eahy 926 cell line under 1% 02 hypoxia. Eahy 926 cells were incubated with HSYA （100, 10 and 1 μmol · L^-1 ） under hypoxia for the indicated time after treatment. Cell proliferation rate was detected using MTT assays. VHL and p53 location and protein expression were analyzed by immunocytochemical stain. HIF-1α, VHL and p53 mRNA expression were detected by RT-PCR. Protein expression of HIF-1α, VHL and p53 were assayed by Western blotting method. HSYA at 100 μmol · L^-1 increased Eahy 926 cells proliferation rate under hypoxia. HIF-1α mRNA and protein expression were up-regulated in the presence of HSYA. VHL, p53 mRNA and protein expression decreased significantly after 8 hours of treatment under hypoxia. HSYA protected Eahy 926 cells from hypoxia, and up-regulated HIF-1α expression partially via its inhibition of VHL and p53 expression.