中文摘要：

目的了解山东省不同地区淡色库蚊成蚊对溴氰菊酯的抗药性水平及其kdr等位基因的突变与抗药性水平间的关联情况。方法采用PCR技术,使用特异性引物克隆不同地区野生淡色库蚊成蚊kdr基因序列,并进行基因测序。利用线性回归分析判断其突变情况与抗药性的关联情况。结果成功克隆出kdr等位基因,通过基因序列分析发现在淡色库蚊钠通道第II结构域S6节段1014位点上存在2种突变,即L1014F：TTA突变为TTT,相应的亮氨酸（L）被苯丙氨酸（F）取代;L1014S：TTA突变为TCA,相应的亮氨酸（L）被丝氨酸（S）取代。以kdr基因L1014F和L1014S等位基因频率x与成蚊接触筒的存活率y建立的回归方程分别为y=1.130566x＋0.34394（r2=0.811684,P〈0.05）和y=2.919579x＋1.06308（r2=0.997039,P〈0.05）。结论淡色库蚊溴氰菊酯抗药性与kdr基因突变呈正相关,具体的抗药性机理亟待进一步研究。

英文摘要：

Objective To investigate the deltamethrin resistance of Culex pipiens pallens and its association with the knockdown resistance mutation.Methods kdr gene sequences were isolated from different parts of wild Culex pipiens pallens mosquitoes using specific primers in PCR and they were then sequenced.Linear regression analysis was used to determine the relationship between the mutation and deltamethrin resistance.Results kdr alleles were cloned and sequenced.Analysis of DNA sequences revealed the presence of two point mutations at the L1014 residue in the IIS6 transmembrane segment of vgsc,i.e.,an L1014F TTA mutation to TTT,with the corresponding leucine（L） replaced by phenylalanine（F）,and an L1014S TTA mutations to TCA,with the corresponding leucine（L） replaced by serine（S）.Using the frequency of L1014F and L1014S alleles as x and the survival rate in the mosquito contact tube as y,regression equations were established with y=1.130566x＋0.34394（r2=0.811684,P〈0.05）and y=2.919579x＋1.06308（r2=0.997039,P〈0.05）,respectively.Conclusion Two alternative kdr-like mutations in Culex pipiens pallens were related to a deltamethrin-resistant phenotype.Further studies are needed to determine the specific mechanism of resistance.