中文摘要：

建立并优化了利用气相色谱-质谱（Gas chromatography-mass spectrometry,GC-MS）检测酰基高丝氨酸内酯（Acyl-homoserine lactones,AHLs）的分析方法。通过优化升温程序,采用选择离子检测（m/z 143）,可同时检测7种AHLs（C4、C6、C7、C8、C10、C12和C14）,检出限分别为1.50、2.00、1.50、2.00、2.00、2.50和2.50μg l-1,在2.0 mg l-1浓度范围内均呈线性关系（R2〉0.997）。加标回收率实验表明,采用乙酸乙酯萃取,水中7种AHLs的回收率均在54%~97%之间;不同比例的土水体系中,砖红壤和黄棕壤提取液中AHLs的回收率均在56%~108%之间。不同介质中AHLs回收率与其Log P值（P：AHLs在正丁醇和水溶液中的分配比）及水溶解度对数均显著相关,表明采用乙酸乙酯萃取水和土壤提取液中的AHLs时,回收率主要与AHLs的Log P值和水溶解度有关。采用该方法对土壤提取液中AHLs进行测定发现砖红壤和黄棕壤提取液中7种AHLs的浓度分别为3.8~8.7μg l-1和4.2~9.8μg l-1。因此,不仅对于水溶液,土壤提取液等复杂介质中的AHLs也可以采用乙酸乙酯萃取后GC-MS进行分析测定。

英文摘要：

Quorum sensing, the communication between microorganisms, is mediated by specific diffusible signal molecules. Gram-negative bacteria generally use N-acyl-homoserine lactones （AHLs） as their command language in coor- dinating their intra- and intercellular population behaviors. AI-ILs play an important role in controlling diverse physiological processes, including generation of extracellular toxin and enzymes by pathogenic bacteria, formation of biofilm, biolumi- nescence, biosynthesis of antibiotics, bacteria motility, etc. The authors have found in their recent studies that communi- cation of microbial signals is closely related to microbial degradation of organic pollutant. Therefore, the detection and quantification of AHLs are essential for investigation of quorum sensing in gram-negative bacteria. Currently, there are several AI-ILs detection methods, including mainly enzyme linked immunosorbent assay （ELISA） , biosensor, thin layer chromatography （TLC） and chromatography. In this study, a fast, simple selective method was established for detecting N-acyl-homoserine lactones （AHLs） in soil solution by the GC-MS （gas chromatography-mass spectrometry）. The novel method can detect AHLs qualitatively and quantitatively at the same time. Using the full scan mode, seven kinds of AHLs （ C4 , C6 , C7 , C8 , C10 , C12 and C14-HSL） standards were analyzed by GC-MS. The best results in terms of selectivity and time consumption of the analysis were ob- tained. Sample injection was done in the split mode （5：1） and pure helium （99. 999% ） was used as carrier gas at a flow rate of 1 ml min-1 The GC injector temperature was optimized to 290 ℃. The oven temperature program was optimized to start from 100 ℃ to 150 ℃ at a rate of 35 ℃ min-1 and then to 280 ℃ at a rate of 25 ℃ and maintained for6 min. Under such optimized conditions, all the 7 kinds of AHLs were well separated within 12 minutes. Furthermore, the mass spec- trum of the 7 AHLs exhibited a molecular ion peak [ M ] ?