中文摘要：

通过外加淀粉酶预处理剩余污泥,考察了淀粉酶对污泥的破解效果,研究了接种产氢菌（Enterococcussp.LG1）和未接种产氢菌两种状况下,污泥厌氧发酵产氢效果,并与相应温度（60℃）热预处理污泥的发酵产氢效果进行对比,同时分析探讨了污泥发酵产氢过程中底物和pH值的变化.结果表明,淀粉酶预处理污泥4h后水解效果最佳,溶解性化学需氧量与总化学需氧量之比（SCOD/TCOD）从原污泥的6.36%增加到30.928%,可溶性蛋白质和可溶性糖分别达到原污泥的8.65倍和51.65倍.淀粉酶预处理污泥接种产氢菌（Enterococcussp.LG1）后,产氢效果较好,最大产氢率可达13.92mL·g^-1,为淀粉酶处理污泥未接种产氢菌的1.875倍,60℃热处理污泥接种产氢菌的2.834倍,60℃热处理污泥未接种产氢菌的3.085倍.在总的发酵产氢过程中,可溶性糖的平均利用率高达62.87%,是污泥厌氧发酵过程中的主要营养物质.

英文摘要：

The effects of external enzymes（α-amylase）on excess sludge hydrolysis were investigated.Batch experiments were used to characterize anaerobic fermentative hydrogen production using excess sludge pretreated by α-amylase with and without inoculation of the hydrogen-producing bacterial species,Enterococcus sp.LG1.Comparison was also made between bio-hydrogen production with α-amylase pretreated sludge and 60℃ thermally pretreated sludge.After four hours of hydrolysis with α-amylase addition,SCOD/TCOD rose from 6.36% （raw sludge） to 30.928% and the concentration of soluble protein and carbohydrate reached 8.65 and 51.65 times that of raw sludge respectively.Pretreatment with α-amylase and with Enterococcus sp.LG1 inoculation produced a maximal hydrogen yield （H2/VS） of 13.92 mL·g-1,which was 1.875 times that of sludge pretreated by α-amylase without inoculation,2.834 times that of sludge pretreated by 60℃ heat with inoculation and 3.085 times that of 60℃ heat treated sludge without inoculation.During the process,the average utilization coefficient of soluble carbohydrate reached 62.87%,which indicated that soluble carbohydrate was the dominant substrate for bio-hydrogen fermentation.