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与疾病和重要生理功能相关的蛋白质的结构与功能研究
  • 项目名称：与疾病和重要生理功能相关的蛋白质的结构与功能研究
  • 项目类别：创新研究群体科学基金
  • 批准号：30221003
  • 申请代码：C10
  • 项目来源：国家自然科学基金
  • 研究期限：2003-01-01-2008-12-31
  • 项目负责人：饶子和
  • 负责人职称：教授
  • 依托单位：清华大学
  • 批准年度：2002
中文摘要：

本项目的研究目标是与人类疾病相关的重要蛋白质及其复合物组装体的三维结构及其功能的研究；重要蛋白质的聚集规律和折叠机制的研究以及胚胎发育相关蛋白的表达、结构及其功能的研究，研究成果力争在总体上达到当时国际同类工作的先进水平，某些方面争取占有领先地位，主要研究论文力争达到国际知名学术刊物的发表水平。项目实施六年来在研究群体全体成员的共同努力下，我们圆满完成了预计的研究目标，取得了丰硕的研究成果，发表研究论文90余篇。其中部分研究论文在国际著名学术刊物如Cell、Science、Nature、Developmental Cell、 PNAS、PLos Bio、EMBO.J、Developmental 、J. Biol. Chem.、Journal of Virology等上发表。

中文主题词：
成果综合统计
成果类型
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  • 期刊论文
  • 会议论文
  • 专利
  • 获奖
  • 著作
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期刊论文
Capture of monomeric refolding intermediate of human muscle creatine kinase.
Zebrafish Dpr2 inhibits mesoderm induction by promoting lysosomal degradation of Nodal receptors.
Crystal Structure of Mitochondrial Respiratory Membrane Protein Comples Ⅱ.
Structural insights into coronavirus transcription/replication from the crystal structure of the SAR
Design of Wide-Spectrum Inhibitors Targeting Coronavirus Main Proteases.
Tob1 controls dorsal development of zebrafish embryos by antagonizing maternal ?β-Catenin transcript
Cloning and expression analysis of zebrafish tob1 gene.
Osmophobic effect of glycerol on irreversible thermal denaturation of rabbit creatine kinase.
Unassisted refolding of urea-denatured arginine kinase from shrimp Feneropenaeus chinensis: Evidence
Catalysis of creatine kinase refolding by protein disulfide isomerase involves disulfide cross-link
3D structure of human FK506-binding protein 52: implications for the assembly of the glucocorticoid
Crystal Structure of Human Pirin an iron-binding nuclear protein and transcription cofactor.
Crystal structure of Severe Acute Respiratory Syndrome Coronavirus Spike Protein Fusion Core*
fgf17b, a novel member of Fgf family, helps patterning zebrafish embryos.
Sodium barbital is a slow reversible inactivator of rabbit-muscle creatine kinase.
Identification of the HIV-1 gp41 core-binding motif-HXXNPF.
Antibodies induced by multi-epitope vaccine showed inhibitory activity against heterologous influenz
Crystal structure of human E1 enzyme and its complex with a substrate analog reveals the mechanism o
Repeated epitope in the recombinant epitope-peptide could enhance ELDKWA-epitope-specific antibody r
Cloning, characterization and expression analysis of a calcium channel β subunit from pearl oyster (
Cloning and characterization of an IKK homologue from pearl oyster, Pinctada fucata.
Pf-Rel, a Rel/ nuclear factor-κB homolog identified from the pearl oyster, Pinctada fucata.
Crystal Structure of Human Pyrroline-5-carboxylate Reductase.
Crystal Structures of Human Glycerol 3-phosphate Dehydrogenase 1 (GPD1).
The dodecamer structure of the SARS coronavirus non-structural protein nsp10.
Crystal structure of human transgelin
Surface exposure of the HIV-1 env cytoplasmic tail LLP2 domain during the membrane fusion process: i
Fine-epitope mapping of an antibody that binds the ectodomain of influenza matrix protein .
Interaction of HIV-1 gp41 core with NPF motif in Epsin: implication in endocytosis of HIV.
The inner shell film: an immediate structure participating in pearl oyster shell formation.
Candidate muti-peptide-vaccine against classical swine fever virus induced potent immunity with sero
The neutralizing epitope ELDKWA on HIV-1 gp41:
An SP1-like transcription factor Spr2 acts downstream of Fgf signaling to mediate mesoderm induction
Structural basis for coronavirus-mediated membrane fusion: Crystal structure of mouse hepatitis viru
Crystal structure of the polymerase PA(C)-PB1(N) complex from an avian influenza H5N1 virus
Cloning and expression pattern of Smad3 homolog from the pearl oyster, Pinctada fucata.
Identification of the amino acids crucial for the activities of drought responsive element binding f
Cloning, characterization and expression patterns of three sarco/endoplasmic reticulum Ca2+-ATPase (
The Crystal Structures of SARS Virus Main Protease Mpro and Its Complex with an Inhibitor.
Impact of intra-subunit domain-domain interactions on creatine kinase activity and stability.
Spying the neutralizing epitopes on E2 N-terminal by candidate epitope-vaccines against classical sw
Marker vaccine strategies and candidate CSFV marker vaccines.
Predefined spacers between epitopes on a recombinant epitope-peptide impacted epitope-specific antib
A new anti-viral target revealed by the hexameric structure of the MHV coronavirus non-structural pr
Activation and roles of ALK4/ALK7-mediated maternal TGF? signals in zebrafish embryo.
Amotl2 is essential for cell movements in zebrafish embryo and regulates c-Src translocation.
Assisting the reactivation of guanidine hydrochloride-denatured aminoacylase by hydroxypropyl cyclod
The conserved Ala37 in the ERF/AP2 domain is essential for binding with the DRE element and the GCC
The AKAP Yu is required for olfactory long-term memory formation in Drosophila.
Monomeric creatine kinase aggregation and sodium dodecyl sulfate-cyclodextrin assisted refolding.
A novel extracellular EF-hand protein involved in the shell formation of pearl oyster,
Different effects of Pb2+ and Cu2+ on immune and antioxidant enzyme activities of Pinctada fucata.
Surface simulation synthesis: A new strategy to spy alpha-helix structure.
Characterization of immunity induced by M2e of influenza virus.
Exploration of the polar microenvironment around the reactive cysteine in rabbit muscle creatine kin
Cloning and Characterization of 37 kDa Laminin receptor precursor in pearl oyster, Pinctada fucata.
An insight into the mechanism of human cysteine dioxygenase: Key roles of the thioether-bonded tyros
The crystal structure of human isopentenyl diphosphate isomerase at 1.7 E resolution reveals its cat
Crystal Structure of RS21-C6, Involved in Nucleoside Triphosphate Pyrophosphohydrolysis.
The Crystal Structure of MCAT from Mycobacterium tuberculosis Reveals Three New Catalytic Models.
High epitope density in single protein molecule significantly enhanced antigenicity as well as immun
Structure of human spindlin1: tandem tudor-like domains for cell cycle regulation
Production of Authentic SARS-CoV Mpro with Enhanced Activity: Application as a Novel Tag-cleavage En
Structures of two coronavirus main proteases: implications for substrate binding and antiviral drug
Polyvinyl pyrrolidone 40 assists the refolding of bovine carbonic anhydrase B by accelerating the re
V3 CTL epitope density in a single recombinant molecule antigen differentially affects the number an
Characterization of calcium deposition and shell matrix protein secretion in primary mantle tissue c
Effect of O-superfamily conotoxin SO3 on synchronized spontaneous calcium spikes in cultured hippoca
Molecular Epidemiology and Evolution of Worldwide enterovirus 71 Strains Isolated from 1970 to 2004.
The epitope recognized by a monoclonal antibody in influenza A virus M2 protein is immunogenic and c
smad2 and smad3 are required for mesendoderm induction by transforming growth factor-beta/nodal sign
Characterization of antibody responses against a neutralizing epitope on the glycoprotein E2 of clas
Evidence for existence of a close association between CD14 and CXCR4 on monocytic cell line U937.
Role of osmolytes as chemical chaperones during the refolding of aminoacylase.
Candidate peptide-vaccine induced potent protection against CSFV and identified a principal sequenti
A novel carbonic anhydrase from the mantle of the pearl oyster (Pinctada fucata)
Sequence comparison of the extracellular domain of M2 protein between human and avian influenza A vi
Remarkably high activities of testicular cytochrome c in destroying reactive oxygen species and in t
Regulating the DRE-mediated signaling pathway by synergic functions of trans-active and trans-inacti
1970～2004年全球肠道病毒71型分离株的分子流行病学分析
热休克因子结合蛋白（HSBP1）的表达、纯化、结晶与初步晶体学研究
Preliminary Evaluation of a Candidate Multi-Epitope-Vaccine Against the Classical Swine Fever Virus
Cloning, Characterization, and Expression Analysis of Calreticulin from Pearl Oyster Pinctadafucata
Molecular epidemiology and evolution of worldwide enterovirus 71 strains isolated from 1970 to 2004
Molecular Cloning and Distribution of a Plasma Membrane Calcium ATPase Homolog from the Pearl Oyster Pinctadafucata
流感病毒聚合酶PA亚基与PB1多肽复合体的精细三维结构
著作
《Lehninger生物化学原理》
《酶活性部位的柔性》
饶子和的项目
第四届国际结构基因组学大会
蛋白质剪接及在生物工程中的应用
从分子水平到细胞水平的结构生物学发展战略研究
免疫补体系及有关的蛋白质
　SARS冠状病毒复制、转录的结构机制研究
　SARS冠状病毒复制、转录的结构机制研究
小RNA病毒感染与致病机制的结构基础
期刊论文 1
SARS冠状病毒转录复制机器及分子机制和抗病毒药物设计
期刊论文 13
与疾病和重要生理功能相关的蛋白质结构和功能的研究