心脏的 myocytes 的机械特征上的量的调查有重要生理的意义。基于有弹性的底层技术,这份报纸为高效率的细胞的拖拉恢复开发一套算法。由适用,到磁道的一个基于坡度的数字图象关联方法随机在单个心脏的 myocyte 导致的使变形的底层上散布了 uorescence microbeads,高分辨率的底层排水量地能乐意地被获得。由基于不可分的 Boussinesq 答案使用一个数字算法,房间底层拖拉以一种稳定、可靠的方式被重建。最后,当它遵守 polyacrylamide 橡皮底层,单个心脏的 myocyte 的空间与时间的动力学被调查。
Quantitative investigation on mechanical characteristics of cardiac myocytes has important physiological significance. Based on elastic substrate technique, this paper develops a set of algorithms for high-efficiency cellular traction recovery. By applying a gradient-based digital image correlation method to track randomly distributed fluorescence microbeads on the deformed substrate induced by single cardiac myocyte, high-resolution substrate displacement field can readily be obtained. By using a numerical algorithm based on the integral Boussinesq solution, cell-substrate tractions are reconstructed in a stable and reliable manner. Finally, spatiotemporal dynamics of a single cardiac myocyte is investigated as it adheres to a polyacrylamide elastic substrate.