中文摘要：

应用流式细胞术（FCM）检测旋毛虫感染后小鼠肠系膜淋巴结（MLN）中树突状细胞（DC）上甘露糖受体（MR）的影响。培养小鼠骨髓源树突状细胞（BMDC）并负载旋毛虫排泄/分泌抗原（ES抗原）,FCM检测BMDC上MR的变化情况。结果显示,感染第7天MLN中DC表面MR出现下调,但在14 d后出现上调,差异显著（P〈0.05）。体外实验发现,BMDC负载ES抗原后MR出现下调,直到第48小时出现上调,差异显著（P〈0.05）。本研究证明旋毛虫感染后可以引起树突状细胞上MR的变化,表明MR可能是ES抗原的识别受体。这为研制旋毛虫树突状细胞疫苗提供了支持,并对寄生虫免疫逃避机制的研究提供了思路。

英文摘要：

To investigate the effect of Trichinella spiralis infection on MR of dendritic cell （DC） in mesenteric lymph nodes（MLN）on mouse and with cultivation of mouse bone marrow- derived dendritic cells （BMDC） and pulsed of Trichinella spiralis excretory / secretory （ES） antigens, The change of MR on BMDC was detected by FCM. The results showed that the MR receptor on the dendritic cells of MLN was down regulated in seventh days, but it was up - regulated in 14 days. The difference was significant （P 〈 O. 05）. In virto MR was down regulated and it was up - regulated at 48 h, the difference was significant proved that Trichinella spiralis infection can cause changes MR on dendritic cells and it be the recognition receptor of ES antigen. With the research of dendritic cell vaccine, exp （P eriments showed that 〈0.05） indicated this study support to DC vaccine of Trichinella spiralis and immune escape mechanism research of parasite. . This study that MR may can provides