中文摘要：

探讨枫叶黄酮对脂多糖（Lipopolysaccharide,LPS）诱导的小胶质细胞株BV-2细胞炎性因子释放的抑制作用.用LPS刺激BV-2细胞构建炎症模型,采用免疫荧光双标和RT-PCR方法检测不同浓度枫叶黄酮（5,10,15μmol/L）对细胞炎性蛋白酶诱导型一氧化氮合酶（inducible nitric oxide synthase,iNOS）和环氧合酶-2（cyclooxygenase-2,COX-2）、细胞炎性因子肿瘤坏死因子-α（tumor necrosis factor-α,TNF-α）和白介素-1β（interleukin-1β,IL-1β）、炎性信号蛋白核因子-κB（nuclear factor-κB,NF-κB）蛋白与mRNA的表达变化.结果发现：不同浓度的枫叶黄酮在翻译水平和转录水平上明显抑制了LPS诱导的细胞炎性蛋白酶iNOS和COX-2,细胞炎性因子TNF-α和IL-1β与炎性信号蛋白NF-κB的上调.上述结果表明枫叶黄酮可通过调控LPS诱导的小胶质细胞株BV-2细胞炎性因子释放从而抑制小胶质细胞激活,发挥抗神经炎症的作用.

英文摘要：

To investigate suppressing effects of flavonoids from the leaves of Acer truncatum in the activation od BV-2 microglia,cells were treated with flavonoid（5,10,15μmol/L） prior to LPS exposure,then the effects on the mRNA and protein levels of pro-inflammatory enzymes,inducible nitric oxide synthase（iNOS）,cyclooxygenase-2（COX-2）,pro-inflammatory cytokines,tumor necrosis factor-α（TNF-α）,interleukin-1β（IL-1β） and inflammatory signaling proteins nuclear factor-κB（NF-κB） were analysed by RT-PCR and double-immunofluorescence labeling assay.The results indicate that flavonoid has an effect on the expression of iNOS,COX-2,TNF-α,IL-1β and NF-κB through transcriptional and translational inhibition.Therefore we think the flavonoid can restrain microglial cells activity by means of regulation pro-inflammatory mediators of BV-2 cells that induced by LPS,so it can exert anti-neuroinflammatory actions.