中文摘要：

基于共聚焦检测原理，建立了双波长生物芯片检测系统。采用体全息滤光片，利用其反射衍射和带阻滤光特性，无需切换或改变光路，在同一光路中即可实现Cy3与Cy5荧光双波长扫描检测。提出了基于顺序形态变换的生物芯片荧光点阵图像的网格化处理方法，并对Cy3与Cy5荧光试剂点样玻片进行了扫描实验，对实验所得荧光点阵图像进行了网格化处理。实验结果显示，系统的检测灵敏度达到0．1fluo／μm^2，网格化算法简单、有效。

英文摘要：

A dual-wavelength microarray scanner based on laser confocal principle is constructed to detect two-color （Cy3/Cy5） fluorescence. Volume holographic filters are employed. It is not necessary to switch filters when samples tagged with Cy3 and Cy5 are scanned because of diffraction and band elimination property of volume holographic filter. Filter and gridding of microarray images based on order morphological methods are presented. Experimental result shows that sensitivity of the system is 0. 1 fluo/μm^2. The gridding algorithm is simple and effective.