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中文摘要：
目的：研究siRNA封闭乳腺癌抗原相关基因1（BRCAA1）对乳腺癌细胞MCF-7增殖和瞄基因表达的影响。方法：采用RNAi技术对乳腺癌细胞MCF-7细胞BRCAAl基因进行特异性抑制，用阳离子脂质体与化学合成的Pre—designed antiti BRCAA1 siRNA构建转染复合体，反转染MCF-7细胞株48h后提取总RNA，分为未处理（NT）组、阴性对照组、阳性对照组和BRCAA1组，经反转录荧光实时PCR检测BRCAA1和瞄基因mRNA表达情况；检测细胞增殖抑制率。结果：与阴性对照组相比，siRNA转染MCF-7细胞后实验组BRCAA1基因mRNA水平降低了42．3％；Rb基因表达较之阴性对照组上升了11．1％；实验组MCF-7细胞增殖抑制率为（81．9±6．1）％，抑制作用明显强于对照组（P＜0．05）。结论：BRCAA1基因的封闭明显抑制了MCF-7细胞的增殖，BRCAA1基因与瞄基因可能存在有某种相互拮抗的作用。
英文摘要：
Objective： To investigate the effect of blocking BRCAA1 gene expression with siRNA on the proliferation of tumor cell line MCF-7 and Rb gene expression. Methods： RNAi was employed to specifically knock down BRCAA1 expression. MCF-7 cells were transfected with complexes constructed with lipids and chemically synthesized Pre-designed anti-BRCAA1 siRNAs. The total RNA was isolated and reversely transcribed after 48 h. The expressions of BRCAA1 and Rb mRNA were determined by Real-Time PCR. Results： Compared with negative control, transfected MCF-7 cells had a 42.3% decrease in expression of BRCAA1 mRNA and an 11.1% increase in Rb mRNA expression. The inhibitory rate of MCF-7 cells proliferation was （81.6 ± 6. 1 ）%. Conclusion： There may be some antagonistic effect between BRCAA1 gene and Rb gene in proliferation of tumor ceils, which provides a potential target for anti-tumor gene therapy.
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