中文摘要：

基于总值归一化法,通过顺序形态变换实现双色荧光图像数据的校正,对双通道检测的2种不同波长的Cy3、Cy5荧光图像进行了背景分析和归一化系数计算,数据分析显示双色荧光图像背景分割准确,依据其计算双色荧光校正信号,能消除检测通道（PMT）等因素造成的差异.该方法也适用于多波长荧光数据归一化.

英文摘要：

The differences in labeling efficiency and excited energy between two fluorescent dyes （Cy3, Cy5）, gains and dynamic range between red and green channels result in the systematic variation in dual wavelength microarray scanning. A normalization method with order morphology filter is presented. The background estimations of each spot are done by percentile filtering after fluorescent micoarray gridding which is employed by minimum filtering. The result shows that the ratio of two-color fluorescent intensity could be corrected appropriately.