中文摘要：

为筛选出体外抗病毒效果较好的中药总黄酮成分复方，本试验将经初步筛选出的抗病毒效果较好的淫羊藿总黄酮、黄芩总黄酮、野菊花总黄酮、三七总黄酮4个单味成分进行组方，得到9个中药总黄酮成分复方，分别从安全浓度起倍比稀释5个浓度后，以先加药物后加病毒、先加病毒后加药物、病毒和药物同时加入3种加药方式加入到CEF单层细胞中，通过MTT法观察了9个总黄酮成分复方对NDV感染CEF单层细胞的影响。结果表明，各黄酮成分复方在3种加药方式中均表现一定的抗病毒活性，以D570值和最高病毒抑制率综合评价，淫黄、淫野黄、淫三野黄3个复方的抗病毒效果最好。

英文摘要：

In order to screen ideal flavone prescriptions of antivirus in vitro, nine total flavone ingredients prescriptions were formed based on the four total flavones of EF, BSRF, WDFF, and SF, which showed better antivirus activities described in previous experiment. Every prescription was diluted into five series concentrations from its maximal safe concentration, then, added into CEF monolayer with three ways. MTT colorimetric assay was used to detect the effects of the nine pre- scriptions anti-NDV in CEF. The results indicated that all of the prescriptions displayed antivirus abilities in some degree when treated by the above three treatments. Evaluated by DsTo values and the highest virus inhibition ratio, three prescriptions of epimedium total flavones-baikal skullcap root total flavones（EF-BSRF）,epimedium total flavones-wild dendranthema flower total flavones- baikal skullcap root total flavones（EF-WDFF-BSRE）, epimedium total flavones-sanchi total fla- vones-wild dendranthema flower total flavones-baikal skullcap root total flavones（EF-SF-WDFF- BSRF） showed better antivirus activities.