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中文摘要：
为研制抗1型鸭肝炎病毒（DHV-1）新药成分,用水煎醇沉法提取山豆根多糖（BSRPS）,并采用氯磺酸-吡啶法制备其硫酸化产物山豆根多糖硫酸酯（sBSRPS）,然后运用体外细胞培养法比较研究BSRPS和sBSRPS抗DHV-1感染鸭胚肝细胞的作用。采用直接荧光免疫法比较分析BSRPS和sBSRPS对DHV-1吸附肝细胞作用的差异,并用实时荧光定量PCR法比较分析了最有效药物浓度的BSRPS和sBSRPS对DHV-1在鸭胚肝细胞上的复制、释放的影响。结果表明：BSRPS和sBSRPS都具有较好的体外抗DHV-1的作用,sBSRPS的效果优于BSRPS。sBSRPS能有效抑制DHV-1的体外复制和释放,BSRPS仅能抑制DHV-1的复制。两者对病毒吸附鸭胚肝细胞没有明显的作用。结论：sBSRPS可以作为抗DVH的新药主要成分之一。
英文摘要：
The aim of this paper is to research and develop new antiviral drugs ingredients against duck hepatitis virus type 1（DHV-1）.In this paper,the bush sophora root polysaccharide（BSRPS） was extracted by water-extraction and alcohol-precipitation method,and its sulfated product sulfated bush sophora root polysaccharide（sBSRPS） was prepared by chlorosulfonic acid-pyridine method.Antiviral activity against DHV-1 was studied by duck embryonic hepatocytes（DEH） culture in vitro. Direct immunofluorescence method and real-time polymerase chain reaction（real-time PCR） were applied to study the antiviral mechanism of adsorption,replication and release.The results showed that both BSRPS and sBSRPS could exhibite good anti-DHV-1 activities in vitro and sBSRPS performed more effectively than BSRPS. sBSRPS could inhibit both the replication and release of DHV-1 on DEH,while BSRPS only inhibited the replication.Both BSRPS and sBSRPS didn＇t show any influence on the adsorption of DHV-1.Conclusions：sBSRPS would be expected to exploit into the preliminary ingredient of new-type anti-DHV-1 drug.
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