中文摘要：

背景：氟中毒骨转换过程中转化生长因子β超家族成员参与了骨转换过程,但是机制不清楚。目的：观察氟对体外培养成骨细胞中转化生长因子β1,骨形态发生蛋白2和SMAD4信号转导通路的影响。方法：体外培养人成骨细胞,按染氟（NaF）剂量将成骨细胞分为0（对照）,0.625,1.25,2.5,5,10,20,40,80,160mg/L组进行实验观察。结果与结论：氟对成骨细胞增殖影响与转化生长因子β1,骨形态发生蛋白2表达有密切的关系。当氟质量浓度为0.625mg/L时,氟化物产生的效应主要通过转化生长因子β1,骨形态发生蛋白2,SMAD4信号转导通路调节。当低于或超过此剂量时,氟化物产生的效应与信号转导通路关系不大,可能存在转化生长因子β1及骨形态发生蛋白2其他传导通路的调节。

英文摘要：

BACKGROUND：Transforming growth factor（TGF）beta superfamily members are involved in bone turnover process in fluorosis,but the mechanism remains poorly understood.OBJECTIVE：To investigate the effects of fluoride on TGF-β1,BPM-2 and SMAD4 signal transduction pathway of osteoblasts cultured in vitro.METHODS：Human osteoblasts were cultured in vitro and then grouped according to（NaF）dose used：0（control）,0.625,1.250,2.500,5.000,10.000,20.000,40.000,80.000,160.000 mg/L.Cells were collected after 24 hours of culture,RNA extracted,and the mRNA expression of TGF-β1,BPM-2 and SMAD4 was detected using fluorescent quantitative reverse transcription polymerase chain reaction.RESULTS AND CONCLUSION：Fluoride may affect mRNA expression of TGF-β1,BPM-2 in osteoblasts and their expression level was related to fluoride concentration.When NaF was 0.625 mg/L,NaF effect on osteoblasts was regulated via TGF-β1,BPM-2 and SMAD4 signal transduction pathway.When NaF was higher or lower than 0.625 mg/L,NaF effect on osteoblasts was not related to TGF-β1,BPM-2 and SMAD4 signal transduction pathway.There may be other transduction pathways involved in this process.