中文摘要：

为探讨RAW264．7诱导后分化的破骨细胞的鉴定方法，选出了最优方案，为破骨细胞的体外功能研究奠定了基础。通过将RAW264．7细胞以5×10^4接种在96孔板，分别与骨磨片共培养和单独培养，并取小鼠重组sRANKL因子以100ng／mL的浓度诱导10d后分别进行形态学和功能学鉴定和检测，HE、甲苯胺蓝、TRAP染色和扫描电镜观察。结果显示，随着诱导时间的延长。多核细胞数量增多。骨吸收陷窝数量增多；各种方法均可鉴定，HE染色、甲苯胺蓝染色鉴定方法简单但结果不可靠：扫描电镜鉴定准确但操作繁琐；TRAP染色价格较昂贵但结果特异性强、结果准确。研究表明，从形态学角度鉴定TRAP染色为最优，从功能学角度鉴定扫描电镜为最优。

英文摘要：

This paper studies the identification method by inducing RAW264.7 into osteoclast, to find the best procedure, for in vitro functional researches, as a preferable preosteoclast model in respect of its osteoclast characteristic gene expression profile. The inoculate RAW264.7 in 96 well plates, cultured with the bone ground section, of a concentration of 100ng/mL, was used to induce mouse sRANKL for 10 days, which was then examined with respect to morphology and functional identification and detection, using HE staining, Toluidine Blue staining, TRAP staining and SEM observation. It is concluded that with the increase of the induced time, the number of polykaryocyte ceils and the amount of Howship＇s lacuna increase. Among all kinds of methods of identification, HE staining and Toluidine Blue staining are simple but not reliable; the SEM observation is accurate , but complex; and TRAP staining is accurate and shows strong specificity, but is expensive. The results show that the TRAP staining is the best method in view of morphology identification, SEM observation is the best method in view of functional identification. RANKL can induce RAW26d.7 cell into mature osteoclast for morphology and functional identification and detection, which can serve as a basis for our future experimental research about mature osteoclast.