中文摘要：

为了筛选适合用于小鼠不同发育时期胚胎的玻璃化冷冻方法,采用EFS20/40、EFS40、DAP213和GP25法分别冷冻保存不同发育期的小鼠胚胎。结果表明,采用EFS20/40法冷冻的2细胞期胚经解冻后发育至扩张囊胚的比率（93%）显著高于EFS40、DAP213和GP25法（分别为70.9%、39.1%和11.1%）（P〈0.01）;采用EFS20/40法冷冻保存小鼠4～8细胞期胚时,与EFS40相比,解冻后发育至扩张囊胚的比率差异不显著（分别为86.2%和90.0%）,但与DAP213（64.5%,P〈0.05）和GP25法（47.8%,P〈0.01）相比其扩张囊胚率显著增高。此外,虽然EFS40法与EFS20/40法冷冻保存小鼠桑椹胚解冻后的扩张囊胚率差异不显著（分别为68.9%和70.8%）,但均显著高于DAP213（38.9%）和GP25法（37.5%）（P〈0.01）。结果表明,EFS20/40法适合用于小鼠的2细胞期冷冻保存,而EFS20/40和EFS40法均适合用于4～8细胞期胚和桑椹胚的冷冻。

英文摘要：

Different developmental stages of mouse embryos were vitrified using EFS20/40,EFS40,Nakagata-DAP213 or Schefen-GP25 method respectively to filter the most suitable vitrification scheme. The results showed that the blastocyst formation rate by EFS20/40 method （93%） was significantly higher than those by EFS40,Nakagata-DAP213 and Schefen-GP25 methods （70.9%：39.1%：11.1%）after vitrication–thawing 2-cell embryos. After vitrification–thawing 4~8cell embryos,the blastocyst formation rates were not significantly different between EFS20/40 and EFS40 methods（86.2%：90.0%）,but that by EFS20/40 method was better than that by the method of DAP213（64.5%;P0.05）or-GP25（47.8%;P0.01）. In addition,blastocyst formation rates were not significantly different between EFS20/40 and EFS40 methods （68.9%：70.8%）,but they were significantly higher than the methods of Nakagata-DAP213 and Schefen-GP25 after vitrification–thawing Morula embryos（38.9%：37.5%;P0.01）.These results indicated that EFS20/40 method was the most suitable for the 2-cell stage mice cryopreservation,and EFS 20/40 or EFS 40 methods were suitable for 4~8-cell stage embryo and morula vitrification.