中文摘要：

为了探明雌性动物死亡后GV期卵的可利用性，本研究将ICR．系雌性小鼠处死，尸体在4—6℃下分别保存16h、24h和48h，取其卵巢GV期卵，体外成熟后，应用常规法进行体外受精或透明带切割法体外受精，获得2细胞期胚，通过体外培养或胚胎移植观察其发育能力。结果显示，4～6℃下保存16h和24h处理组的体外受精率（分别为31％、33％和21％、24％）与来自新鲜的带有颗粒细胞卵的体外受精率（33％）相比无显著差异。与其相比，保存48h处理组的GV卵已丧失发育能力。此外，体外受精中获得的2细胞胚经体外培养，16h处理组和24h处理组的囊胚率（60％、61％和72％、83％）与新鲜GV期卵处理组的囊胚率（72％）相比差异不显著。获得的2细胞胚经胚胎移植可得到正常幼鼠。上述结果表明，如果雌性小鼠死亡后立即在冷藏温度下（4～6℃）保存，其体内的GV卵在24h以内仍保持发育能力。

英文摘要：

This study was carried out to investigate whether the GV oocytes from carcasses ovarian could be utilized. Carcasses were preserved at 4℃ for 0 hour （ as control）, 16 hours, 24 hours or 48 hours respectively after mice were killed, and GV occytes were collected. After maturating in vitro, these oocytes were fertilized in vitro with the general procedure or cut zona pellucida artificially in advance. 2-cell embryos were transplantated, then viability was observed. The results showed as follows. The fertilizing rate in vitro of the group which conserved 16 hours and 24 hours （31%, 33% and 21%, 24% respectively） was no significant difference with the group of control （33 % ）. Compared with the control group, the GV oocytes which were treated 48 hours had lost their ability to development. Furthermore, the blastula rates of 16 hours and 24 hours group（60% , 61% and 72% , 83% ） , compared with the control group（72% ）, the difference was not significant（P 〉 0. 05 ）. Preservation time or zona pellucida cut could effect fertilizing rate and blastula rate. After some of the 2-cell em- bryos were transplantated, normal young mice could be obtained. The results demonstrated that the female mice were immediately preserved at refrigeration temperature after the death, these GV oocytes could keep these ability of development within 24 hours.