中文摘要：

信号变换器的背景激活和抄写 6 的使活跃之物(STAT6 ) 在 Th_2 依赖的过敏症正式就职的迟了的阶段起一个关键作用。STAT6 对 Th_2 房间区别，招募，和受动器功能必要。我们的以前的学习证实 DNA 种痘禁止了变应原导致的怒气房间的 STAT6 表示。Inthe 现在的学习，我们决定了是否编码 Dermatophagoides pteronyssinus 组 2 的 DNA 疫苗(Der p2 ) 能下面调整在从气喘的老鼠的肺织物的 STAT6 的表示和激活。在 DNA 疫苗的免疫以后的方法， BALB/c 老鼠被 intraperitonealinjection 敏化并且由 rDer p2 的 intranasal 灌输质问了。在 BAL 液体的 cytokines IL-4 andIL-13 的层次被连接酶的免疫吸着剂试金测量。肺织物被免疫估计与 anti-STAT6 的组织化学的染色。STAT6 的蛋白质表示被西方的污点决定。STAT6 有约束力的能力的激活与 electrophoretic 活动性移动试金被分析。结果 DNA 疫苗编码 Der p2 变应原有效地减少了在气喘的老鼠的 IL-4and IL-13 的层次。组织学的证据和西方的污点证明在 DNA 的 STAT6 的表示对待老鼠显著地被稀释。对在从基因的肺织物的特定的 DNA 主题有约束力的 STAT6 种牛痘鼠标被禁止。结论 DNA 疫苗编码 Der p2prevents 过敏肺的发炎可能与 Der p2 在老鼠由禁止 STAT6 表明小径导致变应原的过敏航线发炎。

英文摘要：

Background Activation of signal transducer and activator of transcription 6 （STAT6） plays a critical role in the late phase of Th2-dependent allergy induction. STAT6 is essential to Th2 cell differentiation, recruitment, and effector function. Our previous study confirmed that DNA vaccination inhibited STAT6 expression of spleen cells induced by allergen. In the present study, we determined whether DNA vaccine encoding Dermatophagoides pteronyssinus group 2 （Der p2 ） could down-regulate the expression and activation of STAT6 in lung tissue from asthmatic mice. Methods After DNA vaccine immunization, BALB/c mice were sensitized by intmperitoneal injection and challenged by intranasal instillation of rDer p2. The levels of the cytokines IL-4 and IL-13 in BAL fluid were measured by enzyme-linked immunosorbent assay. The lung tissue was assessed by immunohistochemical staining with anti-STAT6. The protein expression of STAT6 was determined by Western blot. The activation of STAT6 binding ability was analyzed with electrophoretic mobility shift assay. Results DNA vaccine encoding Der p2 allergen effectively decreased the levels of IL-4 and IL-13 in the asthmatic mice. Histological evidence and Western blot showed that the expression of STAT6 in the DNA treated mice was markedly attenuated. STAT6 binding to specific DNA motif in lung tissue from the gene vaccinated mice was inhibited. Condusion DNA vaccine encoding Der p2 prevents allergic pulmonary inflammation probably by inhibiting the STAT6 signaling pathway in mice with Der p2 allergen-induced allergic airway infammafion.