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中文摘要：
通过对纯培养的粉尘螨提取总RNA，采用RT—PCR方法有效地扩增出Dern8片段，将Derf18连接到pET-24a表达载体上并转化到表达菌B121（DE3）中，得到重组质粒pET-Derf18和重组工程菌。重组工程菌经IPTG诱导培养，高效表达出Derf18蛋白，SDS—PAGE结果显示表达产物约为52kDa。表达产物经亲和层析纯化，用Westernblot方法检测免疫学活性，结果表明目的蛋白具有良好的免疫原性。
英文摘要：
In this study, the total RNA of live mites Dermatophagoidesfarinae were isolated and used for RT-PCR amplification of the Der f18 gene. The cloned Der f18 was ligated imo expression vector pET-24a and then transformed into E. coli BI21 （DE3） cells for expression. Expression of recombinant Der f18 protein was induced by adding IPTG to the growing E. coli BL21 （DE3） cells which contained the constructed plasmid. SDS-PAGE analysis showed that the mass weight of the recombinant Der f18 protein was about 52 kDa. IgE reactivity of recombinant Der f18 protein was investigated by immunoblot and the positive results indicate that the recombinant protein have good immunogenicity.
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