中文摘要：

利用表面等离子体共振技术对屋尘螨重组变应原（rDer p2）与单克隆抗体之间的结合作用进行了研究,并且运用该技术对尘螨过敏患者血清与变应原之间的结合作用进行了初步探讨。变应原和抗体之间的结合通常是通过免疫吸附技术或者酶免法来进行研究的,这些方法一般需要对分析物进行标记,由于背景的影响和低灵敏度,目前对抗原和抗体的反应检测所用的免疫吸附技术的准确性还远不如表面等离子共振技术。用氨基偶联法将重组变应原rDer p2固定在CM5传感片表面上后,表面等离子体共振（SPR）技术检测的结果显示,rDer p2与尘螨过敏患者血清的结合作用比单克隆抗体要弱得多。而且,尘螨过敏不同患者血清与rDer p2的结合有很大的差异性。本研究为临床上对过敏疾病的诊断提供了一个简单、快速和实时监控的检测手段。

英文摘要：

In the present paper, the authors aimed to detect the binding of monoelonal antibody and polyclonal antibodies in sera of patients with recombinant house dust mite allergen （rDer p2） by surface plasmon resonance （SPR） technique. This technique is superior to other methods, like enzyme-linked immunosorbent assays （ELISAs） in studying the interaction between biomolecules because no labeling and sample separation are needed. The allergen rDer p2 was immobilized on carboxymethyldextranmodified sensor chip surface by amine coupling. Surface plasmon resonance measurements of monoclonal antibody and polyclonal antibodies in patients＇ sera revealed that their bindings diverge widely; the binding of patients＇ sera was remarkably lower than that of monoclonal antibody. At the same time, the binding of patients＇ sera with rDer p2 varied among patients allergic to dust mite. This study could provide an easy, fast and real-time way for clinical allergic diseases diagnosis.