中文摘要：

目的探讨IgA／VCA抗体滴度与EBVLMP2特异性细胞免疫水平和EBVDNA拷贝数关系。方法免疫酶法检测普查样本IgA／VCA抗体滴度；选取IgA／VCA抗体阳性人群和治疗前鼻咽癌患者，通过γ-干扰素Elispot和EBVDNA荧光定量试剂盒检测LMP2特异性细胞免疫水平和血浆中的EBVDNA载量。结果普查广西苍梧县城自然人群共1233人，其中80人IgA／VCA抗体阳性，阳性率为6．0％。对其中的72例IgA／VCA抗体阳性人群和20未治疗的鼻咽癌患者进行了LMP2特异性细胞免疫和EBVDNA检测，随着抗体滴度的升高，LMP2特异性细胞免疫反应水平降低，而血浆中EBVDNA拷贝数有随IgA／VCA抗体滴度升高而增加的趋势。结论EBV—LMP2特异性细胞免疫水平和EBVDNA拷贝数可能与IgA／VCA抗体滴度相关。

英文摘要：

Objective To investigate the correlation between EBV-LMP2-specific cell responses, EBV DNA copies and titers of IgA antibody to viral capsid antigen （IgA/VCA）. Methods IgA/VCA antibody were tested with immune-enzyme method. Both of plasma and lymphocytes were respectively separated from IgA/VCA-positive individuals and patients with nasopharyngeal carcinoma （NPC） without treatment. EBV DNA copies and LMP2-specifie cell responses were detected by interferon-gamma Elispots assay and fluorescent quantitative PCR method. Results IgA/VCA antibody in 80 of 1 233 individuals was positive in Cangwu county, and the positive rate is 6%. EBV-LMP2-specific cell responses and EBV DNA copies were detected in 72 of 80 IgA/VCA positive individuals and patients with NPC. EBV DNA copies in plasma were increased with rise of titers of IgA/VCA antibody, while EBV-LMP2-specific cell responses were declined. Conclusion EBV-LMP2-specific cell responses and EBV DNA copies are probably associated with titers of IgA/VCA antibody.