中文摘要：

目的：观察细胞外调节蛋白激酶1/2（ERK1/2）、磷酸化细胞外调节蛋白激酶1/2（p-ERK1/2）和基质金属蛋白酶9（MMP-9）在鼻咽癌（NPC）组织中的表达及相关性，并探讨其意义。方法采用免疫组织化学染色SP法检测75份NPC组织（ NPC组）和27份鼻咽黏膜慢性炎组织（炎性组）中ERK1/2、p-ERK1/2和MMP-9蛋白的表达，分析三者在NPC组织中表达的相关性。结果 NPC组ERK1/2、p-ERK1/2和MMP-9蛋白阳性表达率均显著高于炎性组（P均＜0．01），χ2值依次为19．8、17．1、21．3；NPC组织中ERK1/2、p-ERK1/2和MMP-9蛋白三者间的表达呈正相关（P均＜0．01），其中ERK1/2与p-ERK1/2、MMP-9蛋白的r值分别为0．58、0．34，p-ERK1/2、MMP-9蛋白的r值为0．34。结论 ERK1/2、p-ERK1/2和MMP-9蛋白在NPC组织中呈高表达且三者间呈正相关，p-ERK1/2可能通过调节MMP-9表达参与NPC侵袭、转移。

英文摘要：

Objective To investigate the expression changes of extracellular signal-regulated kinase 1/2 （ERK1/2）, phosphorylated extracellular signal-regulated kinase 1/2 （ p-ERK1/2 ） and matrix metalloproteinase 9 （ MMP-9 ） in naso-pharyngeal carcinoma （NPC） and their correlations, and to discussed the significance.Methods The expression of ERK1/2, p-ERK1/2 and MMP-9 proteins was detected in 75 cases of NPC tissues （ NPC group） and 27 cases of chronic nasopharyngeal inflammation tissues （CNI group） by immunohistochemical SP method , and the relationships among them in NPC tissues were analyzed .Results The positive expression rates of ERK 1/2, p-ER-K1/2 and MMP-9 proteins of the NPC group were significantly higher than those of CNI group （all P〈0.01, χ2 =19.8, 17.1 and 21.3, respectively）. The expression of p-ERK1/2 and MMP-9 was positively correlated with ERK1/2 （r=0.58,0.34 respectively, both P〈0.01）, p-ERK1/2 and MMP-9 expression was positively correlated with each other （r=0.34, P〈0.01）.Conclusion ERK1/2, p-ERK and MMP-9 proteins are highly expressed in human NPC and is positively correlated with each other .The p-ERK1/2 may participate in the invasion and metastasis of NPC by regulating the expression of MMP-9.