中文摘要：

有特定的教材的量的实时反向抄写的聚合酶链反应(qRT-PCR ) 试金由世界健康组织推荐了() 广泛地为察觉成功地被使用了并且流行 H1N1/2009 监视流行性感冒一个病毒。在这研究，我们报导在 qRT-PCR 试金要使用检测流行 H1N1/2009 病毒的教材的一个新奇集合的设计和描述。最新设计的教材指向高度在红血球凝聚素之中被保存的三个区域(哈) 流行 H1N1/2009 病毒的基因并且与谁推荐教材指向的那些不同。有最新设计的教材的 qRT-PCR 试金为检测流行 H1N1/2009 病毒和包括人，猪，和浣熊狗起源的流行性感冒 B 病毒和流行性感冒 A 病毒的另外的流行性感冒病毒高度象谁推荐教材一样特定、特定。而且，有最新设计的教材的 qRT-PCR 试金与我们的教材作为试金的察觉限制与谁推荐教材显得是至少 10 褶层比那些更敏感，谁推荐教材分别地每反应是目标 RNA 的 2.5 和 25 个拷贝。当与 83 件临床的样品测试了时， 32 被检测用有我们的设计教材的 qRT-PCR 试金积极，当时仅仅 25 由有谁推荐教材的试金是积极的。这些结果建议有最新设计的教材的 qRT-PCR 系统为流行 H1N1/2009 病毒感染的诊断代表高度敏感的试金。

英文摘要：

A quantitative real time reverse-transcription polymerase chain reaction （qRT-PCR） assay with specific primers recommended by the World Health Organization （WHO） has been widely used successfully for detection and monitoring of the pandemic H1N1/2009 influenza A virus. In this study, we report the design and characterization of a novel set of primers to be used in a qRT-PCR assay for detecting the pandemic H1N1/2009 virus. The newly designed primers target three regions that are highly conserved among the hemagglutinin （HA） genes of the pandemic HlN1/2009 viruses and are different from those targeted by the WHO-recommended primers. The qRT-PCR assays with the newly designed primers are highly specific, and as specific as the WHO-recommended primers for detecting pandemic H1N1/2009 viruses and other influenza viruses including influenza B viruses and influenza A viruses of human, swine, and raccoon dog origin. Furthermore, the qRT-PCR assays with the newly designed primers appeared to be at least 10-fold more sensitive than those with the WHO-recommended primers as the detection limits of the assays with our primers and the WHO-recommended primers were 2.5 and 25 copies of target RNA per reaction, respectively. When tested with 83 clinical samples, 32 were detected to be positive using the qRT-PCR assays with our designed primers, while only 25 were positive by the assays with the WHO-recommended primers. These results suggest that the qRT-PCR system with the newly designed primers represent a highly sensitive assay for diagnosis of the pandemic HIN1/2009 virus infection.